Mycopathologia

, Volume 79, Issue 3, pp 153–158 | Cite as

Circulating immune complexes and in vitro cell reactivity in paracoccidioidomycosis

  • M. Arango
  • F. Oropeza
  • O. Anderson
  • C. Contreras
  • N. Bianco
  • L. Yarzàbal

Abstract

The severest forms of paracoccidioidomycosis (Pcm) are associated with impaired cell-mediated immunity, a phenomenon that is reversible with therapy. It has been postulated that plasma factors could be responsible for such immune dysfunction. In this report, circulating immune complexes (CIC) were measured by the Raji cell radioimmunoassay (Raji) and by the125I-C1q binding assay (CIq-BA) in sera from 14 patients with either active or inactive forms of Pcm and from 15 healthy controls. The C1q-B A revealed significantly elevated levels of CIC in the sera of all but one of the patients. Four of the 8 active (62%) and 2 of the 6 inactive (33%) patients had CIC levels significantly higher than the controls as determined by the Raji test. Significantly increased levels of CIC were detected only in the active patients by the Raji test. The serum of one of the patients, with a generalized infection and depressed lymphocyte responsiveness, was examined and found to contain a factor which depressed the in vitro proliferation of both homologous and normal lymphocytes. We also found that pre-culture of the patients’ lymphocytes before stimulation restored their proliferative capacity, and IC were detectable in the culture supernatants. However, the subsequent addition of the patients’ serum to such precultured cells did not reinduce the depression. It is suggested therefore, that the depression of T cell responses observed in Pcm is due to the presence of IC which may interact reversibly with the responding cells and/or activate a suppressor cell population whose activity is diminished by preculture.

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References

  1. 1.
    Arango, M. & Yarbàzal, L. A., 1982. T.cell dysfunction and hyperimmuno glubulinemia E in paracoccidioidomycosis. Mycopathologia 79: 115 123.PubMedGoogle Scholar
  2. 2.
    Mok, P. W. Y. & Greer, D. L., 1977. Cell-mediated immune response in patients with paracoccidioidomycosis. Clin. Exp. Immunol. 28: 89–98.PubMedGoogle Scholar
  3. 3.
    Musatti, C. C., Rezkallah, M. T., Mendes, E. & Mendes, N. F., 1976. In vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidioidomycosis. Cell. Immun. 24: 365–378.CrossRefGoogle Scholar
  4. 4.
    Paganelli, R., Ngu, J. L. & Levinsky, R. J., 1980. Circulating immune complexes in onchocerciasis. Clin. Exp Immunol., 39: 570–575.PubMedGoogle Scholar
  5. 5.
    Pérez-Rojas, G., Suàrez-Chacón, R., Penchaszadeh, G., Tapanes, F., Contreras, C. E., Abadi, I., Pinto, A., Armas, P., Zschaeck, D., Boissiere, M. & Bianco, N. Pathophysiology of the immune response in systemic lupus erythematosus: a new approach. In: Streptococcal disease and the immune response. Ed. by S. E. Read & J. B. Zabriskie, Academic Press, pp. 507.Google Scholar
  6. 6.
    Restrepo, A., Restrepo, M., Restrepo, F., Aristizabal, L. H., Moncada, L. H. & Velez, H., 1978. Immune responses in paracoccidioidomycosis. A controlled study of 16 patients before and after treatment. Sabouraudia. 16: 151–163.PubMedGoogle Scholar
  7. 7.
    Theofilopoulos, A. N., Wilson, C. B. & Dixon, F. J., 1976. The Raji Cell radioimmune assay for detecting immune complexes in human sera. J. Clin. Inv. 57: 169–182.CrossRefGoogle Scholar
  8. 8.
    Yoshinoya, S., Cox, R. E. & Pope, R. M., 1980. Circulating immune complexes in coccidioidomycosis. Detection and characterization. J. Clin. Inv. 66: 655–663.CrossRefGoogle Scholar
  9. 9.
    Zubler, R. H., Lange, G., Lambert, P. H. & Miescher, P. A., 1976. Detection of immune complexes in unheated sera by a modified125I-Clq binding test. Effect of heating on the binding of C1q by immune complexes and application of the test to systemic lupus erythematosus. J. Immunol. 116: 231–138.Google Scholar

Copyright information

© Dr. W. Junk Publishers 1982

Authors and Affiliations

  • M. Arango
    • 1
  • F. Oropeza
    • 2
  • O. Anderson
    • 2
  • C. Contreras
    • 2
  • N. Bianco
    • 2
  • L. Yarzàbal
    • 1
  1. 1.Sección de Inmunología Parasitaria, CEPIALET, OPS/OMSInstituto Nacional de Dermatología, U.C.V.Caracas
  2. 2.Centro Nacional de Referencia en Inmunología Clínica, SAS-UCVInstituto Anatomo-Patológieo, U.C.V.Caracas

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