Journal of tissue culture methods

, Volume 8, Issue 1, pp 13–16 | Cite as

The use of stimulated primary spleen cell cultures in evaluating cell cycle response to toxicant insult

  • Jeanne A. Anson
  • William G. Hinson
  • Henry Schol
  • James L. Pipkin


Isolated rodent spleen cells, cultured for 48 hours with the mitogen Concanavalin A (Con A), consistently provide a cycling population with which to test the cell cycle effects (both temporal and quantitative) of various toxicants. Any modification in the cell cycle is then analyzed by flow cytometric techniques and computer-aided statistical comparisons of the DNA histogram of the cell populations.

Key words

spleen cell culture flow cytometry cell cycle 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Pallavicine, M. G.; Gray, J. W.; Folstad, L. Quantitative analysis of the cytokinetic response of KHT tumorsin vivo to 1-beta-D-arabino-furanosylcytosine. Cancer Research 42: 3125–3131; 1982.PubMedGoogle Scholar
  2. 2.
    Collste, L. G.; Darzynkiewicz, Z.; Traganos, F.; Sharpless, T. K.; Sogani, P.; Grabstald, H.; Whitmore, W. F.; Melamed, M. R. Flow cytometry in bladder cancer detection and evaluation using acridine orange metachomatic nuclei acid staining of irrigation cytology specimens. J. Urology 123: 478–485; 1980.Google Scholar
  3. 3.
    Collste, L. G.; Darzynkiewicz, Z.; Traganos, F.; Sharpless, T. K.; Whitmore, W. J.; Melamod, M. R. Identification of polymorphonuclear leukocytes in cytologic samples for flow cytometry. J. Histochem. Cytochem. 27: 390–393; 1979.PubMedGoogle Scholar
  4. 4.
    Hiddeman, W.; Clarkson, B. D.; Buechner, T.; Melamed, M. R.; Androff, M. Bone marrow cell count per cubic millimeter bone marrow: a new parameter for quantitating therapy-induced cytoreduction in acute leukemia. Blood 59: 216–225; 1982.PubMedGoogle Scholar
  5. 5.
    Anson, J. F.; Hinson, W. G.; Schol, H.; Pipkin, J. L.; Hudson, J. L. Flow cytometric cell cycle analysis of immune cell proliferation: Anin vitro immunotoxic screen. In Vitro 19: 246; 1983.Google Scholar
  6. 6.
    Vindelov, Lars L. Flow microfluorometric analysis and nuclear DNA in cells from solid tumors and cell suspension. Virchows Arch. B. Cell Path. 24: 227–242; 1977.Google Scholar
  7. 7.
    Oldham, J. W.; Casciano, D. A.; Farr, J. A. The isolation and primary culture of viable non-proliferating rat hepatocytes. T.C.A. Manual 5(2): 1047–1050; 1979.CrossRefGoogle Scholar
  8. 8.
    Bagwell, C.B.; Hudson, J.L.; Irvin, G.L. III. Nonparametric flow cytometry analysis. J. Histochem. 27: 293–296; 1979.Google Scholar
  9. 9.
    Nomura, T. In vivo cell cycle synchronization of the murine sarcoma 180 by continuous colcemid infusion. Nippon-Siekeigeka-Gakki-Zusshi. 54: 1719–1732; 1980.Google Scholar
  10. 10.
    Bohmer, R. M. Determination of non-proliferating cells in culture by combining flow cytometry with stalhmokinetics. Cell-Tissue-Kinetics 13: 497–503; 1980.Google Scholar
  11. 11.
    Pipkin, J.L.; Anson, J. F.; Hinson, W. G.; Schol, H.; Sheehan, D. M. Spleen cell pre-replicational phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. J. Biochem. 95: 323–333; 1984.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • Jeanne A. Anson
    • 1
  • William G. Hinson
    • 1
  • Henry Schol
    • 1
  • James L. Pipkin
    • 1
  1. 1.Cell Biology Branch (HFT-164)National Center for Toxicological ResearchJeffersonUSA

Personalised recommendations