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Journal of tissue culture methods

, Volume 8, Issue 1, pp 13–16 | Cite as

The use of stimulated primary spleen cell cultures in evaluating cell cycle response to toxicant insult

  • Jeanne A. Anson
  • William G. Hinson
  • Henry Schol
  • James L. Pipkin
Article

Summary

Isolated rodent spleen cells, cultured for 48 hours with the mitogen Concanavalin A (Con A), consistently provide a cycling population with which to test the cell cycle effects (both temporal and quantitative) of various toxicants. Any modification in the cell cycle is then analyzed by flow cytometric techniques and computer-aided statistical comparisons of the DNA histogram of the cell populations.

Key words

spleen cell culture flow cytometry cell cycle 

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Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • Jeanne A. Anson
    • 1
  • William G. Hinson
    • 1
  • Henry Schol
    • 1
  • James L. Pipkin
    • 1
  1. 1.Cell Biology Branch (HFT-164)National Center for Toxicological ResearchJeffersonUSA

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