Breast Cancer Research and Treatment

, Volume 14, Issue 2, pp 235–243 | Cite as

Growth kinetics of four human breast carcinomas grown in nude mice

  • M. Spang-Thomsen
  • K. Rygaard
  • L. Hansen
  • A. -C. Halvorsen
  • L. L. Vindeløv
  • N. Brünner


The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.

Key words

breast cancer cell kinetics flow cytometry growth curves nude mice xenografts 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • M. Spang-Thomsen
    • 1
  • K. Rygaard
    • 1
  • L. Hansen
    • 1
  • A. -C. Halvorsen
    • 1
  • L. L. Vindeløv
    • 2
  • N. Brünner
    • 1
  1. 1.University Institute of Pathological AnatomyCopenhagenDenmark
  2. 2.Finsen InstituteCopenhagenDenmark

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