Growth kinetics of four human breast carcinomas grown in nude mice
The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.
Key wordsbreast cancer cell kinetics flow cytometry growth curves nude mice xenografts
Unable to display preview. Download preview PDF.
- 5.Steel GG: Growth Kinetics of Tumours. Clarendon Press, Oxford, 1977Google Scholar
- 6.Fogh J, Giovanella BC: The Nude Mouse in Experimental and Clinical Research, Academic Press, New York, 1977Google Scholar
- 7.Fogh J, Giovanella BC: The Nude Mouse in Experimental and Clinical Research, Volume 2, Academic Press, New York, 1982Google Scholar
- 12.Brünner N, Spang-Thomsen M, Poulsen HS, Vindeløv LL, Engelholm SA: Characterization of the T60 human breast carcinoma grown in nude mice. In: Rygaard J, Brünner N, Græm N, Spang-Thomsen M (eds) Immune-Deficient Animals in Biomedical Research. Karger, Basel, 1987, pp 340–347Google Scholar
- 16.Rygaard K, Spang-Thomsen M: ‘Growth’ — a computer program for determination of mean growth curves and calculation of response to therapy of solid tumor xenografts. In: Wu B-q, Zheng J (eds) Immune-Deficient Animals in Experimental Medicine. Karger, Basel, 1989, pp 301–306Google Scholar
- 21.Barlogie B, Göhde W, Johnston DA, Smallwood L, Schumann J, Drewinko B, Freireich EJ: Determination of ploidy and proliferation characteristics of human solid tumors by pulse cytophotometry. Cancer Res 38: 3333–3339, 1983Google Scholar
- 25.Christensen IJ, Hartmann NR, Keiding N, Larsen JK, Noer H, Vindeløv L: Statistical analysis of DNA distributions from cell populations with partial synchrony. In: Lutz D (ed) Pulse-Cytophotometry. European Press Medicon, Ghent, 1978, Vol 3, pp 71–78Google Scholar
- 26.Steel GG: Cell loss from experimental tumours. Cell Tissue Kinet 1: 193–207, 1968Google Scholar
- 29.Spang-Thomsen M, Engelholm SA, Vindeløv LL: Proliferation kinetics of human small cell carcinoma of the lung (SCCL) grown in nude mice. AACR, 1986, abstr no 128Google Scholar
- 31.Brünner N, Spang-Thomsen M, Vindeløv L, Nielsen A, Engelholm SA, Svenstrup B: Dose-dependent effect of 17β-estradiol determined by growth curves and flow cytometric DNA analysis of a human breast carcinoma (T61) grown in nude mice. Expl Cell Biol 53: 220–232, 1985Google Scholar
- 32.Raber MN, Barlogie B, Latreille J, Bedrossian C, Fritsche H, Blumenschein G: Ploidy, proliferative activity and estrogen receptor content in human breast cancer. Endocrinol 3: 36–41, 1982Google Scholar