Breast Cancer Research and Treatment

, Volume 10, Issue 1, pp 11–20

Immunolocalization of a human basal epithelium specific keratin in benign and malignant breast disease

  • Shahnaz Hashmi Dairkee
  • Britt Marie Ljung
  • Helene Smith
  • Adeline Hackett
Report

Summary

This report describes the immunocytochemical localization of a human basal- or myoepithelial-specific antikeratin antibody in benign and malignant breast disease. Reactivity patterns with this antibody have demonstrated the lack of myoepithelial or basal epithelial participation in most benign breast specimens examined including those displaying cystic disease, fibrosis, or hyperplasia. However, in specimens of sclerosing adenosis, strong reactivity with the majority of cells in most ducts suggests a major participation of the myoepithelial cell type. Analysis of 118 breast carcinoma specimens has demonstrated strong, homogeneous reactivity in 4% of the specimens, suggesting a role for the basal epithelial cell in malignancy of the human mammary gland and implications for the prognosis of such tumors. Antigenic characterization of the malignant and benign mammary specimens which are uniformly reactive with the antibody has demonstrated the presence of a 51 kd keratin polypeptide not found in the non-reactive specimens.

Key words

benign breast disease immunocytochemistry keratin mammary carcinoma myoepithelium 

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References

  1. 1.
    Linzell JL: Some observations on the contractile tissue of the mammary glands. J Physiol 130: 257–267, 1955PubMedGoogle Scholar
  2. 2.
    Pinkstaff CA: The cytology of salivary glands. Int Rev Cytol 63: 141–261, 1980Google Scholar
  3. 3.
    Warburton MJ, Ferns SA, Rudland PS: Enhanced synthesis of basement membrane proteins during the differentiation of rat mammary tumor epithelial cells into myoepithelial cellsin vitro. Exp Cell Res 137: 373–380, 1982PubMedGoogle Scholar
  4. 4.
    Rudland PS, Gusterson BA, Huges CM, Ormerod EJ, Warburton MJ: Two forms of tumors in nude mice generated by a neoplastic rat mammary stem cell line. Cancer Res 42: 5196–5208, 1982PubMedGoogle Scholar
  5. 5.
    Carter D, Yardley JH, Shelley WM: Lobular carcinoma of the breast: an ultrastructural comparison with certain duct carcinoma and benign lesions. Johns Hopkins Med J 125: 25–43, 1969PubMedGoogle Scholar
  6. 6.
    Ozzello L: Epithelial-stromal junction of normal and dysplastic mammary gland. Cancer 25: 586–600, 1970PubMedGoogle Scholar
  7. 7.
    Ozzello L: Ultrastructure of the human mammary gland. Pathol Ann 6: 1–58, 1971Google Scholar
  8. 8.
    Ahmed A: The myoepithelium in human breast carcinoma. J Pathol 113: 129–135, 1974PubMedGoogle Scholar
  9. 9.
    Gould VE, Miller J, Jao W: Ultrastructure of medullary, intraductal, lobular and adenocystic breast carcinomas. Am J Pathol 78: 401–416, 1975PubMedGoogle Scholar
  10. 10.
    Ohtani H, Sasano N: Myofibroblasts and myoepithelial cells in human breast carcinoma. An ultrastructural study. Virchows Arch A Pathol Anat Histol 385: 247–261, 1980PubMedGoogle Scholar
  11. 11.
    Gould VE, Jao W, Battifora H: Ultrastructural analysis in the differential diagnosis of breast tumors. Pathol Res Pract 167: 45–70, 1980PubMedGoogle Scholar
  12. 12.
    Linzell JL: The silver staining of myoepithelial cells, particularly in the mammary gland, and their relation to the ejection of milk. J Anat 86: 49–57, 1952PubMedGoogle Scholar
  13. 13.
    Van Bogaert LJ, Abarka J, Maldagne P: Appraisal and pitfalls of myoepithelial cells staining by levanol fast cyanine 5RM. Histochem 54: 251–258, 1977Google Scholar
  14. 14.
    Gabbiani G, Csank-Brassert J, Schneeberger JD, Kapanci Y, Trencher P, Holborow EJ: Contractile proteins in human cancer cells. Am J Pathol 83: 457–474, 1976PubMedGoogle Scholar
  15. 15.
    Macartney JC, Trevithick MA, Kricka L, Curran RC: Identification of myosin in human epithelial cancers with immunofluorescence. Lab Invest 41: 437–445, 1979PubMedGoogle Scholar
  16. 16.
    Bussolati G: Actin-rich (myoepithelial) cells in lobular carcinoma in situ of the breast. Virchows Arch B Cell Pathol 32: 165–176, 1980Google Scholar
  17. 17.
    Bussolati G, Botta G, Gugliotta P: Actin-rich (myoepithelial) cells in ductal carcinoma-in-situ of the breast. Virchows Arch B Cell Pathol 34: 251–259, 1980Google Scholar
  18. 18.
    Gusterson BA, Warburton MJ, Mitchell D, Ellison M, Munro-Neville A, Rudland PS: Distribution of myoepithelial cells and basement membrane proteins in the normal breast and in benign and malignant breast disease. Cancer Res 42: 4763–4770, 1982PubMedGoogle Scholar
  19. 19.
    Hyderman E, Steele K, Ormerod MG: A new antigen on the epithelial membrane, its immunoperoxidase localization in normal and neoplastic tissue. J Clin Pathol 32: 35–39, 1979PubMedGoogle Scholar
  20. 20.
    Taylor-Papadimitriou J, Peterson JA, Arklie J, Burchell J, Ceriani RL, Bodmer WF: Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer 28: 17–21, 1981PubMedGoogle Scholar
  21. 21.
    Hilkens J, Buijs F, Hilgers J, Hageman PH, Sonnenberg A, Koldovsky U, Karande K, Van Hoeven RP, Feltkamp C, Von de Rign JM: Monoclonal antibodies against human milk fat globule membranes detecting differentiation antigens of the mammary gland. In: Peeters H (ed) Protides of Biological Fluids, Vol 29. Pergamon Press, Oxford 1982, pp. 813–816Google Scholar
  22. 22.
    Lane EB, Klymkowsky MW: Epithelial tonofilaments: investigating their form and function using monoclonal antibodies. Cold Spring Harbor Symp Quant Biol 46: 387–402, 1981Google Scholar
  23. 23.
    Dairkee SH, Blayney C, Smith HS, Hackett AJ: Monoclonal antibody that defines human myoepithelium. Proc Natl Acad Sci, USA 82: 7409–7413, 1985Google Scholar
  24. 24.
    Caselitz J, Walter B, Wustrow J, Seifert G, Weber K, Osborn M: A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelia, and basal and suprabasal cells in certain hyperplastic tissues. Virchows Arch Pathol Anat 409: 725–738, 1986Google Scholar
  25. 25.
    Dempsey PJ, DeKretser TA, Brown RW, Whitehead RH, Jose DG: A monoclonal antibody CIBr17 recognizes a myoepithelium-specific antigen in human mammary gland. Int J Cancer 37: 857–866, 1986PubMedGoogle Scholar
  26. 26.
    Kohler G, Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497, 1975PubMedGoogle Scholar
  27. 27.
    Gown AM, Vogel AM: Monoclonal antibodies to human intermediate filament proteins. Amer J Pathol 114: 309–321, 1984Google Scholar
  28. 28.
    Moll R, Franke WW, Schiller DL, Geiger B, Krepler R: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors, and cultured cells. Cell 31: 11–24, 1982PubMedGoogle Scholar
  29. 29.
    Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680–685, 1970Google Scholar
  30. 30.
    Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci, USA 76: 4350–4354, 1979Google Scholar
  31. 31.
    Robbins SL: Textbook of Pathology with Clinical Applications. WB Saunders, Philadelphia and London, 1961Google Scholar
  32. 32.
    Hamperl H: The Myothelia (Myoepithelial Cells). Current Topics Pathol 53: 161–220, 1970Google Scholar
  33. 33.
    Azzopardi JG: Problems in Breast Pathology, Vol II. WB Saunders, London, 1979Google Scholar
  34. 34.
    Dairkee SH, Blayney-Moore CM, Smith HS, Hackett AJ: Concurrent expression of basal and luminal epithelial markers in cultures of normal human breast analyzed using monoclonal antibodies. Differentiation 32: 93–100, 1986PubMedGoogle Scholar
  35. 35.
    Wells S, Hasleton PS: A dilutional immunoperoxidase study of proliferative ductal lesions and carcinomata of the breast. Histopathol 5: 517–526, 1981Google Scholar
  36. 36.
    Haagensen DE Jr, Mazoujian G, Dilley WG, Pederson CE, Kister SJ, Wells SA Jr: Breast gross cystic disease fluid analysis, I. Isolation and radioimmunoassay for a major component protein. J Natl Cancer Inst 62: 239–247, 1979PubMedGoogle Scholar
  37. 37.
    Dardick I, Von Nostrand AWP: Myoepithelial cells in salivary gland tumors-revisited. Head and Neck Surg 7: 395–408, 1985Google Scholar
  38. 38.
    Batsakis JG, Kraemer B, Sciubba J: The pathology of head and neck tumors: the myoepithelial cell and its participation in salivary gland neoplasia, part 17. Head and Neck Surg 5: 222–233, 1983Google Scholar
  39. 39.
    Dulbecco R, Allen RW, Bologna M, Bowman M: Marker evolution during the development of the rat mammary gland: Stem cells identified by markers and the role of myoepithelial cells. Cancer Res 46: 2449–2456, 1986PubMedGoogle Scholar
  40. 40.
    Dairkee SH, Mayall BH, Smith HS, Hackett AJ: Monoclonal marker that predicts early recurrence of breast cancer. Lancet i: 54, 1987Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Shahnaz Hashmi Dairkee
    • 1
  • Britt Marie Ljung
    • 2
  • Helene Smith
    • 1
  • Adeline Hackett
    • 1
  1. 1.Peralta Cancer Research InstituteOaklandUSA
  2. 2.Department of PathologyUniversity of California, San FranciscoSan FranciscoUSA

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