Breast Cancer Research and Treatment

, Volume 14, Issue 1, pp 101–105 | Cite as

Histone acetylation decreased by estradiol in the MCF-7 human mammary cancer cell line

  • J. R. Pasqualini
  • P. Mercat
  • N. Giambiagi
Brief communication

Summary

The effect of estradiol (E2) on the [3H]-acetylation of nuclear histones was studied in the MCF-7 human mammary cancer cell line in culture. Cells (~ 108) were incubated with 8 × 10−6M [3H]-acetate in the absence (control) or in the presence of estradiol (10−5–10−8M). After 20 min incubation, the nuclear histones were extracted and separated by electrophoresis, and each histone band was measured and calculated in DPM/mg protein. It was observed that only the H2 + H3 and H4 histones were associated with the [3H]-acetate. Estradiol (10−6–10−8M) provoked a significant inhibition in the incorporation of the acetate. The negative effect, in percentage of the non-treated cell value, was as follows: in E2 (10−6M): − 25 ± 10 (SE) for H2 + H3 and − 26 ± 5 for H4; in E2 (10−7M): − 35 ± 9 and − 39 ± 10; and in E2 (10−8M): − 56 ± 22 and − 30 ± 13 respectively. It is concluded that estradiol has a negative effect in the acetylation of H2, H3 and H4 histones of this mammary cancer cell; no acetylation or effect is observed in H1 histones. The relationship of this finding to the biological responses of the hormone is to be explored.

Key words

human mammary cancer cells MCF-7 nuclear histones estradiol acetylation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Allfrey VG: Molecular aspects of the regulation of eukaryotic transcription: nucleosomal proteins and their postsynthetic modifications in the control of DNA conformation and template function. In: Goldstein L, Prescott DM (eds) Cell Biology, Vol 3. Academic Press, New York, 1980, pp 347–437Google Scholar
  2. 2.
    Libby PR: Histone acetylation and hormone action. Early effects of oestradiol-17β on histone acetylation. Biochem J 130: 663–669, 1972PubMedGoogle Scholar
  3. 3.
    Pasqualini JR, Cosquer-Clavreul C, Vidali G, Allfrey VG: Effects of estradiol on the acetylation of histones in the fetal uterus of the guinea pig. Biol Reprod. 25: 1035–1039, 1981PubMedGoogle Scholar
  4. 4.
    Liew CC, Suria D, Gornall AG: Effects of aldosterone on acetylation and phosphorylation of chromosomal proteins. Endocrinology 93: 1025–1034, 1973PubMedGoogle Scholar
  5. 5.
    Soule HD, Vazquez J, Long A, Albert S, Brennan M: A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 51: 1409–1416, 1973PubMedGoogle Scholar
  6. 6.
    Lippman M, Bolan G, Huff K: The effect of estrogens and antiestrogens in hormone-responsive human breast cancer in long-term tissue culture. Cancer Res 36: 4595–4601, 1976PubMedGoogle Scholar
  7. 7.
    Horwitz KB, Koseki Y, McGuire WL: Estrogen control of progesterone receptor in human breast cancer: role of estradiol and antiestrogen. Endocrinology 103: 1742–1751, 1978PubMedGoogle Scholar
  8. 8.
    Westley B, Rochefort H: A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell 20: 353–362, 1980PubMedGoogle Scholar
  9. 9.
    Edwards DP, Adams DJ, McGuire WL: Specific protein synthesis regulated by estrogen in human breast cancer. J Steroid Biochem 15: 247–259, 1981PubMedGoogle Scholar
  10. 10.
    Sterner R, Vidali G, Allfrey VG: Studies of acetylation and deacetylation in high mobility group proteins. Identification of the sites of acetylation in high mobility group proteins 14 and 17. J Biol Chem 256: 8892–8895, 1981PubMedGoogle Scholar
  11. 11.
    Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970PubMedGoogle Scholar
  12. 12.
    Doenecke D, Gallwitz D: Acetylation of histones in nucleosomes. Mol Cell Biochem 44: 113–128, 1982PubMedGoogle Scholar
  13. 13.
    Lippman ME, Dickson RB, Kasid A, Gelmann E, Davidson N, McManaway M, Huff K, Bronzert D, Bates S, Swain S, Knabbe C: Autocrine and paracrine growth regulation of human breast cancer. J Steroid Biochem 24: 147–154, 1986PubMedGoogle Scholar
  14. 14.
    Dell'Aquila ML, Pigott DA, Bonaquist DL, Gaffney EV: A factor from plasma-derived human serum that inhibits the growth of the mammary cell line MCF-7: Characterization and purification. J Natl Cancer Inst 72: 291–298, 1984PubMedGoogle Scholar
  15. 15.
    Soto AM, Sonnenschein C: Cell proliferation of estrogensensitive cells: the case for negative control. Endocr Rev 8: 44–52, 1987PubMedGoogle Scholar
  16. 16.
    Soto AM, Sonnenschein C.: The role of estrogens on the proliferation of human breast tumor cells (MCF-7). J Steroid Biochem 23: 87–94, 1985PubMedGoogle Scholar
  17. 17.
    Knabbe C, Huff K, Wakefield L, Lippman ME, Dickson RB: Evidence that transforming growth factor beta is a hormonally regulated negative growth factor in human breast cancer cells. Cell 48: 417–428, 1987PubMedGoogle Scholar
  18. 18.
    Butler WB, Kirkland WL, Gargala TL, Goran N, Kelsey WH, Berlinski PJ: Steroid stimulation of plasminogen activator production in a human breast cancer cell line (MCF-7). Cancer Res 43: 1637–1641, 1983PubMedGoogle Scholar
  19. 19.
    Soto AM, Sonnenschein C: Mechanism of estrogen action on cellular proliferation: evidence for indirect and negative control on cloned breast tumor cells. Biochem Biophys Res Commun 122: 1097–1103, 1984PubMedGoogle Scholar
  20. 20.
    Katzenellenbogen BS, Norman MJ, Eckert RL, Peltz SW, Mangel WF: Bioactivities, estrogen receptor interactions, and plasminogen activator-inducing activities of tamoxifen and hydroxytamoxifen isomers in MCF-7 human breast cancer cells. Cancer Res 44: 112–119, 1984PubMedGoogle Scholar
  21. 21.
    Kassis JA, Sakai D, Walent JH, Gorski J: Primary cultures of estrogen-responsive cells from rat uteri: induction of progesterone receptors and a secreted protein. Endocrinology 114: 1558–1566, 1984PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • J. R. Pasqualini
    • 1
  • P. Mercat
    • 1
  • N. Giambiagi
    • 1
  1. 1.C.N.R.S. Steroid Hormone Research UnitFoundation for Hormone ResearchParisFrance

Personalised recommendations