Journal of tissue culture methods

, Volume 9, Issue 2, pp 83–93

Cultivation, frozen storage, and clonal growth of normal human epidermal keratinocytes in serum-free media

  • Steven T. Boyce
  • Richard G. Ham

DOI: 10.1007/BF01797779

Cite this article as:
Boyce, S.T. & Ham, R.G. Journal of Tissue Culture Methods (1985) 9: 83. doi:10.1007/BF01797779


Methods are described for serum-free culture of human epidermal keratinocytes derived from neonatal foreskin tissue. Cultures are initiated, stored frozen, and returned to active growth, all with bovine pituitary extract as the only undefined supplement. Clonal growth assays are then performed in a biochemically defined medium. The degree of stratification and differentiation in the defined medium (and also with pituitary extract) is controlled by the extracellular calcium ion concentration.

Key words

keratinocytes human epidermis serum-free calcium differentiation 

Copyright information

© Tissue Culture Association, Inc. 1985

Authors and Affiliations

  • Steven T. Boyce
    • 1
  • Richard G. Ham
    • 1
  1. 1.Department of MolecularCellular, and Developmental Biology University of ColoradoBoulderUSA

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