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Prolongation of rat pancreatic islet allograft survival by treatment of recipient rats with monoclonal anti-interleukin-2 receptor antibody and cyclosporin


Since interleukin-2-receptor expressing cells play a role in allograft rejection, we investigated the effect of anti-interleukin-2 receptor monoclonal antibody treatment on graft survival of allografted pancreatic islets. When pancreatic islets obtained from Lewis A-rats (haplotype RT1a) were grafted under the kidney capsules of streptozotocin-diabetic Lewis rats (haplotype RT1u), the recipients relapsed into hyperglycaemia within 11 days (7±1 days). Treatment of the recipient rats with low-dose cyclosporin (1.5 mg/kg body weight) had no effect on allograft survival (9±1 days). The application of anti-interleukin-2 receptor monoclonal antibody (1mg/kg body weight) for 10 days resulted in a prolongation of allograft survival (42.5±15.3,p<0.01). In 3 out of 11 animals a permanent normoglycaemia (>120 days) associated with glucose intolerance was observed. When the recipients were treated for 10 days with cyclosporin and anti-interleukin-2 receptor monoclonal antibody, the allograft survival was also prolonged (45.1±14.6,p<0.01); again 3 out of 11 animals remained permanently normoglycaemic while exhibiting a normal glucose tolerance.


  1. 1.

    Diamantstein T, Osawa H (1986) The interleukin-2 receptor, its physiology and a new approach to a selective immunosuppressive therapy by anti-interleukin-2 receptor monoclonal antibodies. Immunol Rev 92: 5–27

  2. 2.

    Lord RH, Hancock WW, Colby AJ, Diamantstein T, Tilney NL (1986) Effects of anti-IL-2 receptor monoclonal antibody on IL-2 receptor positive cells infiltrating cardiac allografts in the rat. XI. International Congress Transplantation Society, Helsinki (Finland) 3.–8.8. 1986 (Abstract)

  3. 3.

    Kupiec-Weglinski JW, Diamantstein T, Tilney NL, Strom TB (1986) Therapy with monoclonal antibody to interleukin-2 receptor spares suppressor T-cells and prevents or reverses acute allograft rejection in rats. Proc Natl Acad Sci USA 83: 2624–2627

  4. 4.

    Osawa H, Diamantstein T (1984) Partial characterization of the putative rat-interleukin-2 receptor. Eur J Immunol 14: 374–376

  5. 5.

    Ziegler B, Hahn HJ, Ziegler M (1985) Insulin recovery in pancreas and host organs of islet grafts. Exp Clin Endocrinol 85: 53–60

  6. 6.

    Hahn HJ (1978) Die isolierte Langerhans'sche Insel, ein Modell zur Untersuchung der Insulinsekretion in vitro. Endokrinologie 71: 308–324

  7. 7.

    Dorn A, Ziegler M, Bernstein HG, Dietz H, Rinne A (1983) Introducing a monoclonal antibody to insulin: the islets of Langerhans as a model for immunocytochemistry. Acta Histochem 73: 293–295

  8. 8.

    Wilke B, Reiher K, Schmidt S, Klöting I (1986) Verlängerung der Überlebenszeit im Lewis-Ratten-System durch Kultivierung oder PUVA-Vorbehandlung der isolierten Inseln. Z Exp Chir Transplant Künstl Organe 19: 249–251

  9. 9.

    Lacy PE, Davic JM (1984) Transplantation of pancreatic islets. Ann Rev Immunol 2: 183–198

  10. 10.

    Diamantstein T, Volk HD, Tilney NL, Kupiec-Weglinski JW (1986) Specific immunosuppressive therapy by monoclonal anti-IL-2 receptor antibody and its synergistic action with cyclosporin. Immunobiology, 172: 391–399

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Hahn, H.J., Kuttler, B., Dunger, A. et al. Prolongation of rat pancreatic islet allograft survival by treatment of recipient rats with monoclonal anti-interleukin-2 receptor antibody and cyclosporin. Diabetologia 30, 44–46 (1987). https://doi.org/10.1007/BF01788907

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Key words

  • Pancreatic islet allograft
  • immunotherapy
  • anti-IL-2 RMAB
  • cyclosporin, graft histology