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Functional aspects of calcium transport in sarcoplasmic reticulum vesicles derived from frog skeletal muscle treated with saponin

Summary

To study the physiological aspects of the excitation-contraction cycle, saponin (10–100Μg ml−1) was used as a skinning agent on muscle and sarcotubular vesicles derived from fast muscles (sartorius and tibialis anterior) ofRana esculenta.

The vesicles showed similar Ca2+-ATPase activity and similar protein profiles carried out by SDS-PAGE. Calcium transport in untreated vesicles and those treated with different concentrations of saponin seemed to have the same quantitative and qualitative parameters if the saponin was used in a range between 10 and 50Μg ml−1.

Our results confirm that saponin may be considered to be a valid skinning agent for the external membranes of fast skeletal muscles.

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Correspondence to G. Fanó.

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Fanó, G., Belia, S., Fulle, S. et al. Functional aspects of calcium transport in sarcoplasmic reticulum vesicles derived from frog skeletal muscle treated with saponin. J Muscle Res Cell Motil 10, 326–330 (1989). https://doi.org/10.1007/BF01758428

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Keywords

  • Calcium
  • Skeletal Muscle
  • ATPase Activity
  • Sarcoplasmic Reticulum
  • Saponin