Zonal differences in nitric oxide synthesis by bovine chrondrocytes exposed to interleukin-1
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To determine the role of nitric oxide (NO) in the inhibition of aggrecan synthesis, we measured levels of NO produced by bovine chondrocytes from different layers of articular cartilage in the presence of interleukin-1 (IL-1). Chondrocytes from the superficial layer showed a large increase in NO synthesis in response to IL-1. Although chondrocytes from the deep layer also produced NO in response to IL-1, the amount was less than that from the superficial layer. Enhanced NO production evoked by IL-1 was accompanied by a significant inhibition of aggrecan synthesis. These data suggest that chondrocytes in both superficial and deep layer of articular cartilage inhibit aggrecan synthesis with IL-1 via NO production. In addition, superficial layer cells respond to lower amounts of IL-1 with respect to NO-production and inhibition of proteoglycan synthesis.
KeywordsCartilage Arthritis Nitric oxide Interleukin-1
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- Arend WP, Dayer JM. Inhibition of the production and effects of interleukin-1 and tumor necrosis factorα in rheumatoid arthritis. Arthr Rheum 1995;38:151–60.Google Scholar
- Van de Loo FS, Joosten LB, Van Lent PM, Arntz OJ, Van den Berg WB. Role of interleukin-1, tumor necrosis factorα, and interleukin-6 in cartilage proteoglycan metabolism and destruction. Arthr Rheum 1995;38:164–72.Google Scholar
- Aydelotte MB, Schumacher BL, Kuettner KE. Heterogeneity of articular cartilage. Articular Cartilage and Osteoarthritis. New York: Raven Press, 1992:237–49.Google Scholar
- Stefanovic-Racic M, Stadler J, Evans CH. Nitric oxide and arthritis. Arthr Rheum 1993;36:1036–44.Google Scholar