Cancer Immunology, Immunotherapy

, Volume 32, Issue 2, pp 117–124 | Cite as

Enhanced tumor susceptibility of immunocompetent mice infected with lymphocytic choriomeningitis virus

  • Markus Kohler
  • Barbara Rüttner
  • Suzanne Cooper
  • Hans Hengartner
  • Rolf M. Zinkernagel
Original articles

Summary

Mice infected i.v. with high doses of lymphocytic choriomeningitis virus (LCMV; 105–106 plaqueforming units) 8–10 days prior to challenge with the methylcholanthrene-induced fibrosarcoma tumor cell line MC57G or the melanoma cell line B16 tumor cells showed an enhanced tumor susceptibility with respect to both growth kinetics of the tumor and the minimal dose necessary for tumor take. After transient initial growth, MC57G tumor cells were all rejected by uninfected C57BL/6 mice by day 14. Mice preinfected i.v. with LCMV 3 weeks before or at the time of tumor challenge, but not those infected 2 months before or 7 days after, showed increasing tumor growth, the tumor take being 100% for 106, 50% for 105 and 37% for 104 MC57G tumor cells injected into the footpad compared with resistance to 106 cells in normal mice. B16 melanoma cells also grew more rapidly in LCMV-preinfected mice and by day 40 tumors were established with about 100 times fewer cells, i.e. about 103 compared with 3×104−3×105 for uninfected mice. Analysis of the growth of tumor cells in normal and in LCMV-carrier mice revealed that the latter mice were not more susceptible to LCMV-infected than to uninfected MC57G. Since LCMV-carrier mice fail to mount LCMV-specific T cell responses, these results suggest that anti-LCMV-specific T cells may be responsible for acquired immunodeficiency hampering immune surveillance against the tumors studied.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Markus Kohler
    • 1
  • Barbara Rüttner
    • 1
  • Suzanne Cooper
    • 1
  • Hans Hengartner
    • 1
  • Rolf M. Zinkernagel
    • 1
  1. 1.Laboratory of Experimental Pathology, Institute of PathologyUniversity of ZurichSwitzerland

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