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Reduced E-cadherin expression correlates with increased invasiveness in colorectal carcinoma cell lines


A reduction in cell adhesiveness and cell invasion are essential steps in tumour progression to metastasis. In the present study two out of seven colorectal carcinoma cell lines exhibited reduced expression of the cell-cell adhesion molecule E-cadherin as assessed by immunofluorescence. The same two cell lines were invasive in the collagen gel and membrane invasion culture system invasion assays. Addition of anti-E-cadherin antibody to a non-invasive carcinoma cell line caused the cells to assume a dissociated morphology on plastic and to become invasive in collagen gels. This demonstrates a causal role for E-cadherin in the maintenance of intercellular adhesion and the suppression of tumour cell invasion and possibly metastasis in colorectal tumour cells.

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  1. 1.

    Takeichi M, 1988, The cadherins cell-cell adhesion molecules controlling animal morphogenesis.Development,102, 639–53.

  2. 2.

    Eidelman S, Damsky CH, Wheelock MJ and Damjanov I, 1989, Expression of the cell-cell adhesion glycoprotein cell CAM 120/80 in normal human tissue and tumours.Am J Pathol,135, 101–10.

  3. 3.

    Shimoyama Y, Hirohashi S, Hirano Set al. 1989, Cadherin cell adhesion molecules in human epithelial tissues and carcinomas.Cancer Res,49, 2128–33.

  4. 4.

    Shimoyama Y and Hirohashi S, 1991, Cadherin intercellular adhesion molecule in hepatocellular carcinoma: loss of E-cadherin expression in an undifferentiated carcinoma.Cancer Lett,37, 131–5.

  5. 5.

    Shimoyama Y and Hirohashi S, 1991, Expression of E- and P-cadherin in gastric carcinomas.Cancer Res,51, 2185–92.

  6. 6.

    Schipper J, Frixen UH, Behrens J,et al. 1991, E-cadherin expression in squamous cell carcinoma of the head and neck: inverse correlation with tumour dedifferentiation and lymph node metastasis.Cancer Res,31, 6329–37.

  7. 7.

    Kinsella AR, Green B, Lepts GC,et al. 1993, The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis.Br J Cancer,67, 904–9.

  8. 8.

    Oka H, Shiozaki H, Kobayashi K,et al. 1993, Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis.Cancer Res,53, 1696–701.

  9. 9.

    Mayer B, Johnson J, Leitl F,et al. 1993, E-cadherin expression in primary and metastatic gastric cancer: down-regulation correlates with cellular de-differentiation and glandular disintegration.Cancer Res,53, 1690–5.

  10. 10.

    Behrens J, Mareel MM, van Roy FM and Birchmeier W, 1989, Dissecting tumour cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion.J Cell Biol,108, 2435–47.

  11. 11.

    Frixen UH, Behrens J, Sachs M,et al. 1991, Ecadherin mediated cell-cell adhesion prevents invasiveness of human carcinoma cells.J Cell Biol,113, 175–85.

  12. 12.

    Mareel MM, Behrens J, Birchmeier W,et al. 1991, Down-regulation of E-cadherin expression in Madin Darby Canine Kidney (MDCK) cells inside tumours in nude mice.Int J Cancer,47, 922–8.

  13. 13.

    Mareel M, Vleminckx K, Vermeulen S,et al. 1992, E-cadherin expression: a counterbalance for cancer cell invasion.Bull Cancer,79, 347–55.

  14. 14.

    Schor SL, 1980, Cell proliferation and migration in collagen substratain vitro.J Cell Sci,41, 159–75.

  15. 15.

    Hendrix MJC, Seftor EA, Seftor REB and Fidler IJ, 1987, A simple quantitative assay for studying the invasive potential of high and low metastatic variants.Cancer Lett,38, 137–47.

  16. 16.

    Brattain MG, Fine WD, Khaled FM,et al. 1987, Heterogeneity of malignant cells from a human colonic adenocarcinoma.Cancer Res,41, 1751–6.

  17. 17.

    Tom BH, Rutzky LP, Jakstys MM,et al. 1976, Human colonic adenocarcinoma cells. I. Establishment and description of a new line.In Vitro,12, 180–91.

  18. 18.

    Hart IR and Saini A, 1992, Biology of tumour metastasis.Lancet,339, 1453–7.

  19. 19.

    Umbas R, Schalken JA, Aalders TW,et al. 1992, Expression of the cellular adhesion molecule Ecadherin is reduced or absent in high-grade prostate cancer.Cancer Res,52, 5104–9.

  20. 20.

    Fidler IJ, Gersten DM and Hart IR, 1978, The biology of cancer invasion and metastasis.Adv Cancer Res,28, 149–250.

  21. 21.

    Vleminckx K, Vakaet LJ, Mareel M,et al. 1991, Genetic manipulation of E-cadherin expression by epithelial tumour cells reveals an invasion suppressor role.Cell,66, 107–19.

  22. 22.

    Chen WC and Obrink, 1991, Cell-cell contacts mediated by E-cadherin (uvomorulin) restrict invasive behaviour of L cells.J Cell Biol,114, 319–27.

  23. 23.

    Doki Y, Shiozaki H, Tahara H,et al. 1993, Correlation between E-cadherin expression and invasivenessin vitro in a human esophageal cancer cell line.Cancer Res,52, 3421–6.

  24. 24.

    Behrens J, Vakaet L, Frius R,et al. 1993, Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the Ecadherin/B-catenin complex in cells transformed with a temperature sensitive v-Src gene.J Cell Biol,120, 757–66.

  25. 25.

    Rubinfeld B, Souza B, Albert I,et al. 1993, Association of the APC gene product with B-catenin.Science,262, 1731–4.

  26. 26.

    Su L-K, Vogelstein B, Kinzler K, 1993, Association of the APC tumour suppressor protein with catenins.Science,262, 1734–7.

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Correspondence to A. R. Kinsella.

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Kinsella, A.R., Lepts, G.C., Hill, C.L. et al. Reduced E-cadherin expression correlates with increased invasiveness in colorectal carcinoma cell lines. Clin Exp Metast 12, 335–342 (1994). https://doi.org/10.1007/BF01753841

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  • E-cadherin
  • invasion assays
  • metastasis