Journal of Muscle Research & Cell Motility

, Volume 7, Issue 2, pp 97–109 | Cite as

The reconstruction of myosin filaments in rabbit psoas muscle from solubilized myosin

  • Maria C. Maw
  • Arthur J. Rowe


Using rabbit psoas muscle strips, A-bands with their myosin-containing thick filaments have been substantially reconstructedin situ (as judged by electron and light microscopy and by low-angle X-ray diffraction analysis) after prior solubilization of the myosin filaments in high ionic strength potassium phosphate solution. The maintenance of a very high local concentration of soluble myosin, by means of a closely apposed artificial semi-permeable membrane is necessary for reconstruction of full-length filaments. This reconstruction effect can be totally abolished by pre-glycerolation of the muscle, or (reversibly) by pre-depletion of Ca2+. Reconstruction at longer sarcomere lengths (>2.6μm) is anomalous, part-length ‘stub filaments’ being formed, with their stub tails projecting out from the I-Z-I lattice. A model is proposed to explain this reconstruction effect.


Ionic Strength Diffraction Analysis Potassium Phosphate Local Concentration High Ionic Strength 
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Copyright information

© Chapman and Hall Ltd 1986

Authors and Affiliations

  • Maria C. Maw
    • 1
  • Arthur J. Rowe
    • 1
  1. 1.Department of Biochemistry, School of Biological SciencesUniversity of LeicesterLeicesterUK

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