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Cytologische und cytogenetische Untersuchungen an homologen und heterologen Lymphocyten-Mischkulturen

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Zusammenfassung

In Mischkulturen von menschlichen Blutlymphocyten verschiedener Spender treten auch in Abwesenheit von Phytohämagglutinin oder von spezifischen Antigenen aktivierte, blastenartige und proliferierende Zellen auf. In Erweiterung bisher vorliegender Befunde wurden Untersuchungen über die gegenseitige Lymphocytenstimulation bei der Kultivierung von Blutzellen anisogenetischer menschlicher Spender und von Milzlymphocyten der Maus und des Meerschweinchens durchgeführt. Die Ergebnisse zeigen, daß die reziproke Lymphocytenaktivierung nicht artbegrenzt ist, sondern auch in Mischkulturen der verschiedenen intra- und interspezifischen Versuchsgruppierungen vorhanden ist. Die in den homologen und heterologen Lymphocyten-Mischkulturen in wechselnder Zahl auftretenden blastenartig transformierten und proliferierenden Zellen zeigen in ihren cytologischfärberischen Eigenschaften eine weitgehende Übereinstimmung mit den unter Phytohämagglutininbzw. spezifischer Antigen-Stimulation nachweisbaren aktivierten Lymphocyten. Cytogenetische Untersuchungsverfahren erlauben mit Hilfe der „Chromosomen-Marker-Technik“ anhand einfacher chromosomaler Erkennungsmerkmale eine Analyse der jeweiligen Herkunft der blastenartigen Zellen und liefern den Beleg dafür, daß die Lymphocyten der Spender-Partner sich gegenseitig zu stimulieren vermögen.

Summary

Blastoid transformation and proliferation of lymphocytes can be observed in mixed cultures of human white blood cells of different donors. This transformation occurs in the absence of phytohaemagglutinin or specific antigens. Observations on reciprocal activation of anisogeneic human lymphocytes of peripheral blood are supplemented by results on mixed cultures of spleen lymphocytes from the mouse and guinea-pig. It should be stressed that reciprocal lymphocytic activation is not limited to a species, but occurs in intra- and interspecific combinations from different donors. This effect can be observed in animals which show no or only a conditioned phytohaemagglutinin-response of lymphocytes. The blastoid cells occuring in homologous and heterologous mixed lymphocyte cultures display the same cytologic characteristics as the human blastoid cells induced by phytohaemagglutinin or antigenic stimulation. Cytogenetic methods provide means of identification of the individual origin of blastoid cells by using simple cytogenetic (chromosomal) markers in the different sexes or species of the donors. Thus it can be stated that mixed lymphocytic reaction leads to a mutual or two-way activation of the lymphocytes of both donors. The mixed lymphocytic reaction seems to represent a immunocytological reaction based on a membrane-bound antigen-antibody interaction of competent lymphocytes.

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Literatur

  1. Bach, F., andK. Hirschhorn: Lymphocyte interaction: A potential histocompatibility test in vitro. Science143, 813–814 (1964).

  2. Bain, B., andL. Lowenstein: Genetic studies on the mixed leukocyte reaction. Science145, 1315–1316 (1964).

  3. Bain, B., M.R. Vas, andL. Lowenstein: The development of large immature mononuclear cells in mixed leukocyte cultures. Blood23, 108–116 (1964).

  4. Desai, R.G., andW.P. Creger: Maternofetal passage of leukocytes and platelets in man. Blood21, 665–673 (1963).

  5. Elrod, L.M., andR. Schrek: Production of lymphoblastoid cells from rat and human lymphocytes by rabbit serum and by phytohemagglutinin. Exp. Cell Res.38, 418–423 (1965).

  6. Elves, M.W., andM.C.G. Israels: Lymphocyte transformation in cultures of mixed leukocytes. A possible test of histocompatibility. Lancet1965 I, 1184–1186.

  7. Elves, M.W., M.C.G. Israels, andM. Collinge: An assessment of the mixed leukocyte reaction in renal failure. Lancet1966 I, 682–685.

  8. Fischer, R., u.A. Gropp: Cytochemie des Lymphocyten in vitro. Klin. Wschr.44, 733–738 (1966).

  9. Fischer, R., A. Gropp u.H. Geiger: Spezifische und unspezifische Lymphocytentransformation in vitro. Verh. dtsch. Ges. Path.50, (1966) (im Druck).

  10. Gropp, A., u.R. Fischer: Ergebnisse der Züchtung von Lymphocyten in vitro. Klin. Wschr.44, 665–671 (1966).

  11. Kadowaki, J.I., R. Thompson, W.W. Zuelzer, P.V. Woolley, A.J. Brough, andD. Gruber: XX/XY lymphoid chimaerism in congenital deficiency syndrome with thymic alymphoplasia. Lancet1965 I, 1152–1155.

  12. Kay, H.E.M., J.H.L. Playfair, M.R. Wolfendale, andP.K. Hopper: Development of the thymus in the human fetus and its relation to immunological potential. Nature (Lond.)196, 238–240 (1962).

  13. Lanman, J.T.: Transplantation immunity in mammalian pregnancy; mechanisms of fetal protection against immunologic rejection. J. Pediat.66, 525–540 (1965).

  14. Marshall, W.H., S.J. Rigo, andS. Melman: Lymphocyte transformation and miosis in vitro initiated by homologous macrophages. Lancet1966 I, 730–732.

  15. Metcalf, W.K.: Some experiments on the phytohaemagglutinin culture of leukocytes from rats and other mammals. Exp. Cell Res.40, 490–498 (1965).

  16. Moorhead, P.S., P.C. Nowell, W.J. Mellman, D.M. Battips, andD.A. Hungerford: Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell Res.20, 613–616 (1960).

  17. Moynihan, P.C., J.F. Jackson, andJ.D. Hardy: Lymphocyte transformation as an in vitro histocompatibility test. Lancet1965 I, 453–455.

  18. Naeslund, J., andG. Nylin: Investigations of permeability of placenta with aid of red blood corpuscles tagged with radioactive phosphorus. Acta med. scand., Suppl.170, 390 (1946).

  19. Nowell, P.C.: Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes. Cancer Res.20, 462–466 (1960).

  20. Rubin, A.L., K.H. Stenzel, K. Hirschhorn, andF. Bach: Histocompatibility and immunologic competence in renal homotransplantation. Science143, 815–816 (1964).

  21. Smith, K., J.L. Duhring, J.W. Greene, D.B. Rochlin, andW.S. Blakemore: Transfer of maternal erythrocytes across the human placenta. Obstet. and Gynec.18, 673–676 (1961).

  22. Williams, T.W., andM. Ray: A method for culturing leucocytes of rats and rabbits. Cytogenetics4, 365–368 (1965).

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Auszugsweise vorgetragen auf der 50. Tagung der Deutschen Gesellschaft für Pathologie in Heidelberg, 1966.

Mit Unterstützung der Deutschen Forschungsgemeinschaft.

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Fischer, R., Gropp, A. & Geiger, H. Cytologische und cytogenetische Untersuchungen an homologen und heterologen Lymphocyten-Mischkulturen. Klin Wochenschr 44, 1366–1371 (1966). https://doi.org/10.1007/BF01747897

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