Cancer Immunology, Immunotherapy

, Volume 34, Issue 3, pp 157–162 | Cite as

Generation of T cell clones binding F(ab′)2 fragments of the idiotypic immunoglobulin in patients with monoclonal gammopathy

  • Anders Österborg
  • Maria Masucci
  • Susanne Bergenbrant
  • Göran Holm
  • Ann-Kari Lefvert
  • Håkan Mellstedt
Original articles
Received:
Accepted:

Summary

Lymphocytes from two patients with multiple myeloma stage I and one patient with monoclonal gammopathy of undetermined significance were found to proliferate specifically in response to low concentrations of F(ab′)2 fragments of the autologous M component. T cell clones isolated from repeatedly stimulated cultures bound specifically the autologous idiotype and proliferated after addition of soluble idiotype and exogenous interleukin-2. The majority of clones were CD8+ and showed negligible staining for CD4. Idiotype-binding clones could not be isolated from cultures of lymphocytes from a healthy control stimulated under the same conditions. The study provides support for the existence of idiotype-reactive T cells in monoclonal gammopathies. Such cells might have a regulatory role on the tumour cell clone and may be important for a future therapeutic approach.

Key words

Monoclonal gammopathy Idiotype T cells 
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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Anders Österborg
    • 1
  • Maria Masucci
    • 2
  • Susanne Bergenbrant
    • 1
  • Göran Holm
    • 1
  • Ann-Kari Lefvert
    • 1
  • Håkan Mellstedt
    • 1
  1. 1.Department of Oncology (Radiumhemmet), Department of Medicine, Immunological Research Lab.Karolinska HospitalStockholmSweden
  2. 2.Department of Tumour BiologyKarolinska InstituteStockholmSweden

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