Cassava-fermenting organisms

  • Donald Nwankwo
  • Edith Anadu
  • Ralph Usoro
Research Papers


Organisms isolated during the fermentation of cassava tubers, as practised for ‘fufu’ production, includedBacillus subtilis, Pseudomonas alcaligenes, Lactobacillus plantarum, Corynebacterium manihot, Leuconostoc mesenteroides andPseudomonas aeruginosa. All isolates displayed amylase activity. Optimum growth and amylase activities of isolated organisms was at 42°C and between pH values 5 and 6. Isolated organisms also displayed similar patterns of antibiotic resistance. Plasmids purified from isolates could not be transferred or maintained inEscherichia coli RR1 cells.

Microorganismes fermentant le manioc


La fermentation des tubercules de manioc a été effectuée comme on le pratique traditionnellement pour la production de ‘fufu’. On a utilisé quatre variétés différentes de tubercules de manioc. Au cours du processus de fermentation, un grand nombre d'espèces microbiennes agissent de concert pour dégrader l'amidon contenu dans les tubercules. Les glycosides cyanogènes, linamarine et lotaustraline sont également degradés. Les microorganismes isolés au cours de la fermentation des tubercules de manioc, comme on la pratique pour la production de ‘fufu’, comprennentBacillus subtilis, Pseudomonas alcaligenes, Lactobacillus plantarum,Corynebacterium manihot, Leuconostoc mesenteroides andPseudomonas aeruginosa. Tous les microorganismes isolés révèlent une activité amylolytique. Les croissances et activités amylolytiques des microorganismes isolés montrent un optimum à 42°C et un pH entre 5 et 6. Les organismes isolés exhibent aussi des profils semblables de résistance aux antibiotiques. Les plasmides purifiés des isolats n'ont pas pu être transférés ni maintenus dans des cellules d'Escherichia coli RR1.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abe, M. O. &Lindsay, R. C. (1978) Evidence for a lactic streptococcal role in Nigerian acid cassava (Manihot esculenta Crantz) fermentations.Journal of Food Protection 41, 781–784.Google Scholar
  2. Akinrele, I. A. (1964) Fermentation of cassava.Journal of Science, Food and Agriculture 15, 589–594.Google Scholar
  3. Anon. (1980)11TA Research Highlights for 1979. Ibadan. ISS No. 331–4340, pp. 32–33.Google Scholar
  4. Birnboim, H. C. &Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Research 7, 1513–1523.Google Scholar
  5. Bolivar, F. &Backman, K. (1979) Plasmids ofEscherichia coli as cloning vectors. In Wu, R. (ed.)Methods in Enzymology, Vol. 68, pp. 245–267. Academic Press, New York.Google Scholar
  6. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlack, M. C., Heynecker, H. L., Boyer, H. W., Crosa, J. H. &Falkow, S. (1977) Construction and characterization of new cloning vehicles II. A multipurpose cloning system.Gene 2, 95–113.Google Scholar
  7. Castric, P. A. (1975) Hydrogen cyanide, a secondary metabolite ofPseudomonas aeruginosa.Canadian Journal of Microbiology 21, 613–618.Google Scholar
  8. Cohen, S. N., Chang, A. C. Y. &Hsu, L. (1972) Non chromosomal antibiotic resistance in bacteria: Genetic transformation ofEscherichia coli by R-factor DNA.Proceedings of the National Academy of Science USA 69, 2110–2114.Google Scholar
  9. Collard, P. &Levi, S. (1959) A two stage fermentation of cassava.Nature 183, 620–621.Google Scholar
  10. Colman, A., Byers, M. J., Priminose, S. B. &Lyons, A. (1978) Rapid purification of plasmid DNAs by hydroxyapatite chromatography.European Journal of Biochemistry 91, 303–310.Google Scholar
  11. Cowan, S. T. &Steel, K. J. (1974)Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge. Cambridge University Press.Google Scholar
  12. Delange, F.,Bourdoux, P.,Camus, M.,Gerard, M.,Mafuta, M.,Hanson, A. &Ermans, M. (1976) The toxic effect of cassava on human thyroid. InProcedings of the 4th Symposium of the International Society for Tropical Root Crops, 237–242.Google Scholar
  13. Henner, D. J. &Hoch, J. A. (1980) TheBacillus subtilis chromosome.Microbiological Reviews 44, 57–82.Google Scholar
  14. Ketiku, A. O. &Oyenuga, V. A. (1970). Preliminary report on the carbohydrate constituents of cassava root and yam tuber.Nigerian Journal of Science 4, 25–30.Google Scholar
  15. Knowles, C. J. (1976) Micro-organisms and cyanide.Bacteriological Reviews 40, 652–680.Google Scholar
  16. Linton, A. H. (1978) Antibiotics, animals and man —an appraisal of a contentious subject. In Woodbine, W. (ed.)Antibiotics and Antibiosis in Agriculture, pp. 315–343. Butterworths, London.Google Scholar
  17. Mielenz, J. R. (1983)Bacillus stearothermophilus contains a plasmid borne gene for α-amylase.Proceedings of the National Academy of Science USA 80, 5975–5979.Google Scholar
  18. Ngaba, P. R. &Lee, J. S. (1979) Fermentation of cassava (Manihot esculenta Crantz).Journal of Food Science 44, 1570–1571.Google Scholar
  19. Okafor, N. (1977) Micro-organisms associated with cassava fermentation for garri production.Journal of Applied Bacteriology 24, 279–284.Google Scholar
  20. Okafor, N. (1983) Indgenous fermented foods.Nigerian Institute of Food Journal 1, 33–37.Google Scholar
  21. Onwueme, I. C. (1978)The Tropical Tuber Crops, pp. 109–148. John Wiley & Sons, London.Google Scholar
  22. Rodgers, P. B. &Knowles, C. J. (1978) Cyanide production and degradation during growth ofChromobacterium violaceum.Journal of General Microbiology 108, 261–267.Google Scholar
  23. Smith, H. W. (1978) Antibiotic resistance in bacteria and associated problems in farm animals before and after the 1969 Swann Report. In Woodbine, M. (ed.)Antibiotics and Antibiosis in Agriculture, pp. 345–357. Butterworths, London.Google Scholar
  24. Yamaguchi, K., Nagata, Y. &Maruo, B. (1974) Isolation of mutants defective in α-amylase fromBacillus subtilis: Genetic analyses.Journal of Bacteriology 119, 416–424.Google Scholar

Copyright information

© Oxford University Press 1989

Authors and Affiliations

  • Donald Nwankwo
    • 1
  • Edith Anadu
    • 1
  • Ralph Usoro
    • 1
  1. 1.Department of Biological SciencesUniversity of CalabarCalabarNigeria

Personalised recommendations