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Time course of rise of muscle stiffness at onset of contraction induced by photorelease of ATP

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Summary

Isometric contraction (9–10°C) of skinned fibres from rat psoas muscle was elicited by photorelease of ATP (1.3–1.4 mM), and force (F), in-phase (I) and quadrature (Q) stiffness were monitored. The sinusoidal length change for the stiffness measurement was ≈ 0.09% fibre length and 200–1000 Hz. On photolysis,F andI initially fell, and then rose. The half-time of the rise ofI was shorter than that ofF. Contrary to the previous assumption,Q rose even earlier thanI. Although the time courses of the rise ofF andI were greatly affected by the presence of phosphate ion (Pi; 4 mM), its effect onQ was modest. We propose the following hypothesis: the rigor crossbridges, after binding ATP, initially enter a state characterized by highQ with lowI and none or littleF, and then, releasing Pi during passing the state with the increasedI, reach the final force generating state.

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Correspondence to K. Horiuti.

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Horiuti, K., Sakoda, T. & Yamada, K. Time course of rise of muscle stiffness at onset of contraction induced by photorelease of ATP. J Muscle Res Cell Motil 13, 685–691 (1992). https://doi.org/10.1007/BF01738257

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Keywords

  • Phosphate
  • Fibre Length
  • Isometric Contraction
  • Length Change
  • Psoas Muscle