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Journal of Muscle Research & Cell Motility

, Volume 13, Issue 4, pp 406–419 | Cite as

Interpretation of the X-ray diffraction pattern from relaxed skeletal muscle and modelling of the thick filament structure

  • S. B. Malinchik
  • V. V. Lednev
Papers

Summary

The first part of this paper is devoted to the model-building studies of our high resolution meridional X-ray diffraction patterns (in the region from 1/500 to 1/50 Å−1) obtained from relaxed frog muscle. A one-dimensional model of thick filament was proposed which basically consists of two symmetrical arrays of 50 crossbridge crown projections. In the proximate and central zones of the filament the crossbridge crowns are regularly shifted with a 429 Å period and appear as triplets with a 130 Å distance between crowns, while the crowns in the distal parts of filament are regularly ordered with a 143 Å repeat. The centre-to-centre distance between regions with crossbridge perturbations is 7050 Å. The length of each crown projection is about 125 Å. The model includes also (1) C-protein component represented in each half of the filament by seven stripes of about 350 Å long and located 429 Å apart, (2) a uniform density of filament backbone of about 1.5 μm length, and (3) 13 high density stripes in a central zone located with 223 Å period. The final model explains very well the positions and intensities of the main meridional reflections. A three-dimensional model of crossbridge configuration is described in the second part of the work. The model was constructed by using the intensity profiles of the first six myosin layer lines of the X-ray pattern from stretched muscle and taking into account the crossbridge perturbations and the axial size of crossbridge crown obtained from the one-dimensional studies. It was found that both myosin heads are tilted in opposite directions along the filament and wrap around the filament backbone. This crossbridge configuration corresponds well to the findings of Haselgrove. To take into account interference effects between thick filaments we proposed a model of the filament hexagonal lattice with disorder of the second kind. The cross-sectional width of crystalline domains in stretched muscle was estimated to be about 0.11 μm. The models presented may be helpful in the interpretation of the X-ray diffraction patterns from contracting muscle.

Keywords

Central Zone Myosin Head Contracting Muscle Layer Line Thick Filament 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • S. B. Malinchik
    • 1
  • V. V. Lednev
    • 1
  1. 1.Institute of Biological PhysicsAcademy of SciencesPushchinoRussia

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