Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Expression of 87A, 87C and 93D heat shock puffs in trisomic (2L and3L) strains ofDrosophila melanogaster: Evidence for a functional relation between 93D and 87C


Chromosomal RNA synthesis of the three heat shock puffs 87A, 87C and 93D examined in trisomic3L (T3L) and trisomic2L (T2L) strains ofDrosophila melanogaster and compared with that of Oregon R + and compound stocks of the3rd [C(3L) VGI ru st; F(3R) VDI e s/F(3R) VDI es] and2nd [C(2L) SHI+; F(2R) bw] chromosomes. The activity at two different temperatures (22° and 37° C) was monitored by [3H]uridine autoradiography. The results that while in the controlT3L andT2L nuclei (22° C), the 93D puff shows a higher level of transcriptional activity than in the Oregon R+ or compound stocks used as controls, inT3L andT2L nuclei from heat-shocked sets (37° C), 93D does not show further induction compared with heatshocked controls, and the 87C puff is 2.8 times more active than the 87A puff. These results suggest that there may be a direct functional and/or regulatory relationship between the activity of 93D and certain sequence specific activity of 87C, and that an optimum level of activity delimits the activity of the heat shock puff 93D inD. melanogaster.

This is a preview of subscription content, log in to check access.


  1. Ashburner M (1970) Patterns of puffing activity in salivary gland chromosomes of Drosophila. V. Response to environmental treatment. Chromosoma 31:356–376

  2. Ashburner M, Bonner JJ (1979) The induction of gene activity in Drosophila by heat shock. Cell 17:241–254

  3. Devlin RH, Grigliatti TA, Holm DG (1985) Gene dosage compensation in trisomics of Drosophila melanogaster. Dev Genet 6:39–58

  4. Fritz-Earle M, Holm DG (1976) The application of compound autosome to insect control including the first experimental success with compound-fragment combinations. In: White D (ed) Proc 15th Int Congr Entomol Wash, Entomol Soc Am, Washington DC, pp 146–156

  5. Ghosh AK (1987) Gene activity in aneuploid genome of Drosophila melanogaster. Ph.D. thesis, Calcutta University, pp 1–133

  6. Ghosh S, Mukherjee AS (1988) Benzamide induced activity patterns of 93D and other puff sites in Drosophila melanogaster with low and high salt. Indian J Exp Biol 26:258–267

  7. Ish-Horowicz D, Pinchin SM, Gausz J, Gyurkovics H, Bencze G, Goldschmidt-Clermont M, Holden JJ (1979) Deletion mapping of two D. melanogaster loci that code for the 70 000 dalton heat induced protein. Cell 17:565–571

  8. Lakhotia SC, Mukherjee AS (1969) Chromosomal basis of dosage compensation in Drosophila. I. Cellular autonomy of hyperactivity of male X chromosome in salivary glands and sex differentiation. Genet Res 14:137–150

  9. Lakhotia SC, Mukherjee AS (1970) Activation of a specific puff by Benzamide in D. melanogaster. Dros Inf Serv 45:108

  10. Lakhotia SC, Mukherjee T (1982) Absence of novel translation products in relation to induced activity of 93D puff in Drosophila melanogaster. Chromosoma 85:369–374

  11. Lakhotia SC, Mukherjee T (1984) Specific induction of the 93D puff in polytene nuclei of Drosophila melanogaster by colchicine. Indian J Exp Biol 22:67–70

  12. Lakhotia SC, Singh AK (1982) Conservation of 93D puff of D. melanogaster in different species of Drosophila. Chromosoma 86:265–278

  13. Lakhotia SC, Singh AK (1985) Non-inducibility of the 93D heat shock puff in cold-reared larvae of Drosophila melanogaster. Chromosoma 92:48–54

  14. Lindsley DN, Grell ER (1972) Genetic variations of Drosophila melanogaster. Carnegie Inst Wash Publ

  15. Lis JT, Prestige L, Hogness DS (1978) A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster. Cell 14:901–919

  16. Mukherjee T, Lakhotia SC (1979)3H-uridine incorporation in the puff 93D and in chromocentric heterochromatin of heat shocked salivary glands of Drosophila melanogaster. Chromosoma 74:75–82

  17. Ritossa F (1964) Experimental activation of specific loci in polytene chromosomes of Drosophila. Exp Cell Res 35:601–607

  18. Ryseek RP, Walldorf U, Hovemann B (1985) Two major RNA products are transcribed from heat locus 93D of D. melanogaster. Chromosoma 93:17–20

Download references

Author information

Correspondence to A. S. Mukherjee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ghosh, A.K., Mukherjee, A.S. Expression of 87A, 87C and 93D heat shock puffs in trisomic (2L and3L) strains ofDrosophila melanogaster: Evidence for a functional relation between 93D and 87C. Chromosoma 99, 71–75 (1990). https://doi.org/10.1007/BF01737291

Download citation


  • Heat Shock
  • Transcriptional Activity
  • Developmental Biology
  • Optimum Level
  • Uridine