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DMNH — Ein neuer kompetenter Indikator für die Messung der Chemilumineszenz von neutrophilen Granulocyten und Monocyten

DMNH — A new sensitive indicator for measuring the chemiluminescence of granulocytes and monocytes

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Summary

Comparative studies on the effects of the luminescence indicators 5-Amino-2,3-dihydro-1,4-phthalazinedione (Luminol, LUM) and 7-Dimethylamino-naphthalene-1,2-dicarbonic-acidhydracide (DMNH) in measuring the chemiluminescence of ingesting and non-ingesting polymorphonuclear leukocytes (PMN) and monocytes (MOC) are described. DMNH is more effective than LUM in measuring chemiluminescence of PMN and MOC under both biological conditions. Indications are given that LUM inhibits the oxidative metabolism of both cell types. The use of DMNH as a luminescence indicator makes possible the research of cells with low activity of the oxidative metabolism in small numbers.

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Abbreviations

CL:

Chemilumineszenz

DMNH:

7-dimethylamino-naphthalin-1,2-dicarbonsäure-hydrazid

DMSO:

Dimethylsulfoxid

LUM:

Luminol, 5-amino-2,3-dihydro-1,4-phthalazinedion

MOC:

Monocyten

PBS:

phosphatgepufferte NaCl-Lösung (p h 7.2)

PMN:

neutrophile Granulocyten

RLU:

relative Lichteinheiten

Literatur

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    Allen RC, Loose LD (1976) Phagocytic activation of a Luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem Biophys Res Commun 69:245–252

  2. 2.

    Allen RC, Stjernholm RL, Steele RH (1972) Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun 47:679–684

  3. 3.

    Eschenbach C (1982) Luminol Chemilumineszenz phagocytierender neutrophiler Granulocyten. Klin Wochenschr 60:357–364

  4. 4.

    Eschenbach C, Hahm W, Adrian U (1985) deDMNH-Chemilumineszenz ingestierender Monocyten. Klin Wochenschr 63:364–370

  5. 5.

    Wulff K (1984) Persönliche Mitteilung

  6. 6.

    Wulff K, Allen RC (1983) Chemiluminigenic probing of phagocyte oxygenation activity. Topics in Biochemistry, Boehringer, Mannheim

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Additional information

Die Untersuchungen wurden mit Sachbeihilfen der Deutschen Forschungsgemeinschaft durchgeführt (Es 26/5-1)

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Eschenbach, C., Adrian, U. DMNH — Ein neuer kompetenter Indikator für die Messung der Chemilumineszenz von neutrophilen Granulocyten und Monocyten. Klin Wochenschr 63, 1218–1225 (1985). https://doi.org/10.1007/BF01733781

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Key words

  • Chemiluminescence
  • Luminescence indicator
  • DMNH
  • Luminol
  • Neutrophils
  • Monocytes