Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate

  • 11 Accesses

  • 15 Citations


Several problems regarding the protein acceptor of the oligosaccharide from GEA (glucosylated endogenous acceptor) were investigated in the present work using rat liver microsomal subfractions. It was found that the acceptor molecule is present in rough and smooth liver microsomes. Furthermore both fractions have closely similar specific activities. The problem of whether nascent peptides must be ribosome bound for glycosylation to occur was studied. The results suggests that binding of peptides to ribosomes is not a necessary condition for the transfer of GEA oligosaccharide to protein. The increase in specific activity found after partial release of the microsomal vesicular content suggests that the acceptor protein for GEA is membrane bound. Evidence obtained in attempting to elucidate whether nascent or completed chains are glycosylated favours the later possibility.

This is a preview of subscription content, log in to check access.


  1. 1.

    Behrens, N. H. and Leloir, L. F., 1970. Proc. Nat. Acad. Sci. U.S. 66, 153–159.

  2. 2.

    Behrens, N. H., Carminatti, H., Staneloni, R. J., Leloir, L. F. and Cantarella, A. I., 1973. Proc. Nat. Acad. Sci. U.S. 70, 3390–3394.

  3. 3.

    Parodi, A. J., Behrens, N. H., Leloir, L. F. and Carminatti, H., 1972. Proc. Nat. Acad. Sci. U.S. 69, 3268–3272.

  4. 4.

    Pless, D. D. and Lennarz, W. J., 1975. J. Biol. Chem. 250, 7014–7019.

  5. 5.

    Hsu, A., Baynes, J. W. and Heath, E. C., 1974. Proc. Nat. Acad. Sci. U.S. 71, 2391–2395.

  6. 6.

    Nikaido, H. and Hassid, W. Z., 1971. Adv. Carbohyd. Chem. and Biochem. 26, 351–483.

  7. 7.

    Melchers, F., 1973. Biochem. 12, 1471–1475.

  8. 8.

    Molnar, J. and Sy, D., 1967. Biochem. 6, 1941–1947.

  9. 9.

    Schachter, H., Jabbal, I., Hudgin, R. L., Pinteric, L., McGuire, E. J. and Roseman, S., 1970. J. Biol. Chem. 245, 1090–1100.

  10. 10.

    Redman, C. M. and Cherian, G., 1972. J. Cell Biol. 52, 231–245.

  11. 11.

    Tabora, E., 1975. Ph.D. Thesis. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires.

  12. 12.

    Sunshine, G. H., Williams, D. J. and Rabin, B. R., 1971. Nature New Biol. 230, 133–136.

  13. 13.

    Adelman, M. R., Blobel, G. and Sabatini, D. D., 1974. Methods in Enzymol. 31, 201–215.

  14. 14.

    Kreibich, G. and Sabatini, D. D., 1974. Methods in Enzymol. 31, 215–225.

  15. 15.

    Vassalli, P., Lisowka, K., Bernstein, B. and Lamm, M. E., 1971. J. Mol. Biol. 56, 1–19.

  16. 16.

    Parodi, A. J., Staneloni, R., Cantarella, A. I., Leloir, L. F., Behrens, N. H., Carminatti, H. and Levy, J. A., 1973. Carbohydr. Res. 26, 393–400.

  17. 17.

    Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., 1951. J. Biol. Chem. 193, 265–275.

  18. 18.

    Campbell, P. N. and Sargent, J. R., 1967. Techniques in Protein Biosynthesis, vol. 1, pp. 301–303, Academic Press, New York.

  19. 19.

    Behrens, N. H., Parodi, A. J., Leloir, L. F. and Krisman, C. R., 1971. Arch. Biochem. Biophys. 143, 375–383.

  20. 20.

    Redman, C. M. and Sabatini, D. D., 1966. Proc. Nat. Acad. Sci. U.S. 56, 608–615.

  21. 21.

    Adelman, M. R., Sabatini, D. D. and Blobel, G., 1973. J. Cell Biol. 56, 206–229.

Download references

Author information

Additional information

Dedicated to ProfessorLuis F. Leloir on the occasion of his 70th birthday.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Idoyaga Vargas, V., Carminatti, H. Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate. Mol Cell Biochem 16, 171–176 (1977). https://doi.org/10.1007/BF01732058

Download citation


  • Lipid
  • Peptide
  • Sugar
  • Oligosaccharide
  • Liver Microsome