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Protein synthesis in resting and stimulated human lymphocytes

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Summary

The ribosomal profiles in lysates from resting and phytohemagglutinin stimulated human lymphocytes have been analyzed by sucrose gradient centrifugation. The percentage of polyribosomes increased during lymphocyte transformation reaching a maximal value of 60 to 70% of the total ribosomes after 72 hours of mitogen addition. This time period coincides with maximalin vivo protein synthesis. On the other hand, in nonstimulated lymphocytes, about 25% of the ribosomal particles appeared as aggregates, independently of the incubation period.

Experiments performed with homologous cell free systems containing ribosomes and supernatant fluids prepared from unstimulated or activated lymphocytes demonstrate that the mixtures containing both components from stimulated lymphocytes are several fold more active in polypeptide synthesis than the systems which contain ribosomal particles and cell sap from resting cells. Assays carried out with mixtures combining the components from both sources indicate that the increased activity depends on ribosomes as well as on the supernatant fractions.

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References

  1. 1.

    Copper, H. L., 1973. In Effects of Drugs on the Cell Cycle (Zimmerman, A., Padilla, G. and Cameron, I., eds.), pp. 137–160, Academic Press, New York.

  2. 2.

    Kay, J. E., 1968. Eur. J. Biochem. 4, 225–232.

  3. 3.

    Kay, J. E., Ahern, T. and Atkins, M., 1971. Biochim. Biophys. Acta 247, 322–334.

  4. 4.

    Ahern, T. and Kay, J. E., 1973. Biochim. Biophys. Acta 331, 91–101.

  5. 5.

    Kay, J. E., Ahern, T., Lindsay, V. J. and Sampson, J., 1975. Biochim. Biophys. Acta 378, 241–250.

  6. 6.

    Ahern, T. and Kay, J. E., 1975. Exptl. Cell Res. 92, 513–515.

  7. 7.

    Ahern, T., Sampson, J. and Kay, J. E., 1974. Nature 248, 519–521.

  8. 8.

    Cooper, H. L., 1968. J. Biol. Chem. 243, 34–43.

  9. 9.

    Cooper, H. L., 1974. In Methods in Enzymology (Fleischer, S. and Packer, L., eds), Vol. 32B, pp. 633–636, Academic Press, New York.

  10. 10.

    Fridlender, B. R., Medrano, E. and Mordoh, J., 1974. Proc. Nat. Acad. Sci. U.S.A. 71, 1128–1132.

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Dedicated to Professor LUIS F. LELOIR on the occasion of his 70th birthday.

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Burrone, O., Algranati, I.D. Protein synthesis in resting and stimulated human lymphocytes. Mol Cell Biochem 16, 105–110 (1977). https://doi.org/10.1007/BF01732050

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Keywords

  • Sucrose
  • Protein Synthesis
  • Polypeptide
  • Incubation Period
  • Gradient Centrifugation