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Molecular and Cellular Biochemistry

, Volume 13, Issue 3, pp 157–163 | Cite as

3H-testosterone distribution and binding in RAT thymus cells in vivo’

  • A. D. Abraham
  • Georgeta Buga
General and Review Articles a. general articles

Summary

Autoradiography and biochemical investigations showed that [3H]-testosterone where injected intraperitoneally into male white rats was incorporated rapidly into thymus lymphocytes. Thymic cortex contained more silver grains than medulla, and larger lymphocytes were more labelled than medium or small lymphocytes.

Cytosol fraction of thymus cells labelledin vivo with [3H]-testosterone, contained the largest quantity of labelled hormone. A 4S cytosol fraction binds [3H]-testosterone. This could be separated by Sephadex chromatography or by linear sucrose gradient centrifugation. Nuclear extract contained also a small quantity of the labelled hormone.

Keywords

Testosterone Cytosol Fraction Sucrose Gradient Biochemical Investigation Small Lymphocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr. W. Junk b.v. Publishers 1976

Authors and Affiliations

  • A. D. Abraham
    • 1
  • Georgeta Buga
    • 1
  1. 1.Biological Research CentreRomania

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