The uptake and release of131I-labelled diferric transferrin by rat reticulocytes was examined both in vitro and in vivo. Cycle time in vitro was estimated to be 2.5 min in iron-deficient reticulocytes and 2.3 min in phenylhydrazine-produced reticulocytes. In vivo reticulocyte uptake and release of labelled diferric transferrin injected in the iron-deficient rat averaged 1.7 min.
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Bothwell TH, Conrad ME, Cook JD, Fielding J, Hallberg L, Izak G, Layrisse M, Ramsay WNM (1978) The measurement of total and unsaturated iron-binding capacity in serum. Br J. Haematol 38: 281–288
Dautry-Varsat A, Ciechanover A, Lodish HF (1983) pH and the recycling of transferrin during receptor-mediated andocytosis. Proc Natl Acad Sci USA 80: 2258–2262
Finch CA, Huebers HA, Eng M, Miller L (1982) Effect of transfused reticulocytes on iron exchange. Blood 59: 364–369
Huebers HA, Huebers E, Csiba E, Finch CA (1978) Iron uptake from rat plasma transferrin by rat reticulocytes. J Clin Invest 62: 944–951
Huebers HA, Bauer W, Huebers E, Csiba E, Finch CA (1981) The behavior of transferrin iron in the rat. Blood 57: 218–228
Huebers HA, Huebers E, Csiba E, Rummel W, Finch CA (1983) The significance of transferrin for intestinal iron absorption. Blood 61: 283–290
Huebers HA, Csiba E, Huebers E, Finch CA (1985) Molecular avantage of diferric transferrin in delivering iron to reticulocytes: a comparative study. Proc Soc Exp Biol Med 179: 222–226
Iacopetta BJ, Morgan EH, Yeoh GCT (1982) Transferrin receptors and iron uptake during erythroid cell development. Biochim Biophys Acta 687: 204–210
Intragumtornchai T, Huebers HA, Eng M, Finch VA (1988) In vivo transferrin-iron receptor relationships in erythron of rats. Am J Physiol 255: R 326–331
Karin M, Mintz B (1981) Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells. J Biol Chem 256: 3245–3252
Mc Farlane AS (1958) Efficient trace-labeling of proteins with iodine. Nature 182: 53
Morgan EH, Appleton TC (1979) Autoradiographic localization of125I-labeled transferrin rabbit reticulocytes. Nature 223: 1371–1372
Muta K, Nishimura J, Ideguchi H, Umemura T, Ibayashi H (1987) Erythroblast transferrin receptors and transferrin kinetics in iron deficiency and various anemias. Am J Hematol 25: 155–163
Ponka P, Schulman HM (1985) Acquisition of iron from transferrin regulates heme synthesis. J Biol Chem 260: 14717–14721
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Intragumtornchai, T., Huebers, H.A. & Finch, C.A. Transferrin-reticulocyte cycle time in rat reticulocytes. Blut 60, 249–252 (1990). https://doi.org/10.1007/BF01728793
- Transferrin receptors
- Reticulocyte iron uptake