Specific radioimmunoassays were used to quantify two basement membrane components, 7S collagen and laminin P1, in sera of 70 patients suffering from diabetes mellitus types I and II with and without clinical signs of chronic diabetic complications. Serum levels of both antigens were increased in diabetics compared to controls (p<0.001). Concentrations of 7S collagen were significantly different in diabetics with signs of microvascular damage compared to those without small vessel disease (p<0.05). The difference between laminin P1 concentrations in the two groups of diabetics was not significant (p<0.2). The augmented levels of circulating 7S collagen and laminin P1 may reflect alterations of basement membrane metabolism. Thus, the measurement of concentrations of these basement membrane components in serum may be a useful tool for monitoring the development of chronic diabetic complications.
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Camerini-Davalos RA, Velasco C, Glasser M, Bloodworth JMB (1983) Drug induced reversal of early diabetic microangiopathy. New Engl J Med 309:1551–1556
Couchman JR, Höök M, Rees DA, Timpl R (1983) Adhesion, growth and matrix production by fibroblasts on laminin substrates. J Cell Biol 96:177–183
Dixit SN, Stuart JM, Seyer JM, Risteli J, Timpl R, Kang AH (1981) Type IV collagens: isolation and characterization of 7S collagen from human kidney, liver and lung. Coll Rel Res Clin Exp 1:549–556
Engel J, Odermatt E, Engel E, Madri JA, Furthmayr H, Rohde H, Timpl R (1981) Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150:97–120
Engerman RL, Colquhoun PJ (1982) Epithelial and mesothelial basement membranes in diabetic patients and dogs. Diabetologia 23:521–524
Foidart JM, Bere EW, Yaar M, Rennard SI, Gullino M, Martin GR, Katz SI (1980) Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein. Lab Invest 42:336–342
Hasslacher C, Reichenbacher R, Gechter F, Timpl R (1984) Glomerular basement membrane synthesis and serum concentrations of type IV collagen in streptozotocin-diabetic rats. Diabetologia 26:150–154
Högemann B, Voss B, Pott G, Rauterberg J, Gerlach U (1984) 7S collagen: a method for the measurement of serum concentrations in man. Clin Chim Acta 144:1–10
Hogan B (1981) Laminin and epithelial cell attachment. Nature 290:737–738
Kefalides NA, Alper R, Clark CC (1979) Biochemistry and metabolism of basement membranes. Int Rev Cytol 61:167–228
Miettinen M, Foidart J-M, Ekblom P (1983) Immunohistochemical demonstration of laminin, the major glycoprotein or basement membranes, as an aid in the diagnosis of soft tissue tumors. Am J Clin Pathol 79:306–311
Risteli J, Bächinger HP, Engel J, Furthmayr H, Timpl R (1980) 7-S collagen: characterization of an unusual basement membrane structure. Eur J Biochem 108:239–250
Risteli J, Draeger KE, Regitz G, Neubauer HP (1982) Increase in circulating basement membrane antigens in diabetic rats and effects of insulin treatment. Diabetologia 23:266–269
Risteli J, Rohde H, Timpl R (1981) Sensitive radioimmunoassays for 7S collagen and laminin: application to serum and tissue studies of basement membranes. Anal Biochem 113:372–378
Risteli L, Timpl R (1981) Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes. Biochem J 193:749–755
Risteli J, Wick G, Timpl R (1981) Immunological characterization of the 7-S domain of type IV collagen. Coll Res 5:419–432
Rohde H, Bächinger HP, Timpl R (1980) Characterization of pepsin fragments of laminin in a tumor basement membrane. Hoppe Seylers Z Physiol Chem 361:1651–1660
Rohde H, Wick G, Timpl R (1979) Immunochemical characterization of the basement membrane glycoprotein laminin. Eur J Biochem 102:195–201
Sachs L (1978) Angewandte Statistik. Springer, Berlin Heidelberg New York
Szarfman A, Hassell JR, Rohrbach DH, Stanley JR, Martin GR (1982) Components of basement membranes: their properties, functions, and alterations in disease states. In: Kuehn K, Schoene H, Timpl R (eds) New trends in basement membrane research. Raven Press, New York
Timpl R, Risteli J, Bächinger HP (1979) Identification of a new basement membrane collagen by the aid of a large fragment resistant to bacterial collagenase. FEBS Lett 101:265–268
Timpl R, Rohde H, Robey PG, Rennard SI, Foidart J-M, Martin GR (1979) Laminin — a glycoprotein from basement membranes. J Biol Chem 254:9933–9937
Williamson JR, Kilo C (1977) Current status of capillary basement-membrane disease in diabetes mellitus. Diabetes 26:65–75
Supported by grants of the Deutsche Forschungsgemeinschaft (Ho 980/1-1, Ra 255/6-1, Vo 339/1-2)
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Högemann, B., Voss, B., Altenwerth, F.J. et al. Concentrations of 7S collagen and laminin P1 in sera of patients with diabetes mellitus. Klin Wochenschr 64, 382–385 (1986). https://doi.org/10.1007/BF01728188
- Basement membranes
- 7S Collagen
- Laminin P1
- Diabetes mellitus