Advertisement

Comparison of agar dilution, broth dilution, disk diffusion, and the E-test for susceptibility testing of penicillin-susceptible and penicillin-resistantStreptococcus pneumoniae

  • J. Thorvilson
  • P. Kohner
  • N. Henry
  • F. CockerillIII
Notes

Abstract

An evaluation to determine the optimal methods for the in vitro susceptibility testing of 41 clinical isolates and the ATCC 49619 strain ofStreptococcus pneumoniae to penicillin was undertaken. No very major or major interpretive errors were observed with the following test methods and media: agar dilution using either Mueller-Hinton medium with lysed horse blood or Haemophilus test medium; broth dilution using cation-adjusted Mueller-Hinton medium with lysed horse blood, Haemophilus test medium, or Todd-Hewitt medium; and the epsilometer test (E-test) using agar containing Mueller-Hinton medium and 5% sheep blood. The disk diffusion method using agar containing Mueller-Hinton medium and 5% sheep blood agar was an effective screening method, requiring confirmation by a dilution susceptibility test method.

Keywords

Agar Clinical Isolate Susceptibility Testing Screening Method Diffusion Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Friedland IR, McCracken GH Jr: Management of infections caused by antibiotic-resistantStreptococcus pneumoniae. New England Journal of Medicine 1994, 331: 377–382.Google Scholar
  2. 2.
    National Committee for Clinical Laboratory Standards: Method for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A3. NCCLS, Villanova. PA, 1993.Google Scholar
  3. 3.
    National Committee for Clinical Laboratory Standards: Performance standards for disk susceptibility tests. Approved standard M2-A5. NCCLS, Villanova, PA, 1993.Google Scholar
  4. 4.
    National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial susceptibility testing. Informational supplement M100-S5. NCCLS, Villanova, PA, 1994.Google Scholar
  5. 5.
    National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial susceptibility testing. Informational supplement M100-S6. NCCLS, Villanova, PA, 1995.Google Scholar
  6. 6.
    Jorgensen JH, Doern GV, Ferraro MJ, Knapp CC, Swenson JM, Washington JA: Multicenter evaluation of the use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing ofStreptococcus pneumoniae and development of quality control limits. Journal of Clinical Microbiology 1992, 30: 961–966.Google Scholar
  7. 7.
    Jacobs MR, Gaspar MN, Robins-Browne RM, Koornhof HJ: Antimicrobial susceptibility testing of pneumococci. Determination of optimal disk diffusion test for detection of penicillin G resistance. Journal of Antimicrobial Chemotherapy 1980, 6: 53–64.Google Scholar
  8. 8.
    Jorgensen JH, Swenson JM, Tenover FC, Ferraro MJ, Hindier JA, Murray PR: Development of interpretive criteria and quality control limits for broth microdilution and disk diffusion antimicrobial susceptibility testing ofStreptococcus pneumoniae. Journal of Clinical Microbiology 1994, 32: 2448–2459.Google Scholar
  9. 9.
    National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A3. NCCLS, Villanova, PA, 1984.Google Scholar
  10. 10.
    Swenson JM, Hall BC, Thornsberry C: Screening pneumococci for penicillin resistance. Journal of Clinical Microbiology 1986, 24: 749–752.Google Scholar
  11. 11.
    Kohner PC, Rosenblatt JE, Cockerill FR III: Comparison of agar dilution, broth dilution, and disk diffusion testing of ampicillin againstHaemophilus species by using inhouse and commercially prepared media. Journal of Clinical Microbiology 1994, 32: 1594–1596.Google Scholar
  12. 12.
    Clark RB, Giger O, Mortensen JE: Comparison of susceptibility test methods to detect penicillin-resistantStreptococcus pneumoniae. Diagnostic Microbiology and Infectious Disease 1993, 17: 213–217.Google Scholar
  13. 13.
    Marshall KJ, Musher DM, Watson D, Mason EO Jr: Testing ofStreptococcus pneumoniae for resistance to penicillin. Journal of Clinical Microbiology 1993, 31: 1246–1250.Google Scholar
  14. 14.
    Jorgensen JH, Ferraro MJ, McElmeel ML, Spargo J, Swenson JM, Tenover F: Detection of penicillin and extended-spectrum cephalosporin resistance amongStreptococcus pneumoniae clinical isolates by use of the E test. Journal of Clinical Microbiology 1994, 32: 159–163.Google Scholar
  15. 15.
    Macias EA, Mason EO Jr, Ocera HY, LaRocco MT: Comparison of E test with standard broth microdilution for determining antibiotic susceptibilities of penicillin-resistant strains ofStreptococcus pneumoniae. Journal of Clinical Microbiology 1994, 32: 430–432.Google Scholar

Copyright information

© MMV Medizin Verlag GmbH Munchen 1997

Authors and Affiliations

  • J. Thorvilson
    • 1
  • P. Kohner
    • 1
  • N. Henry
    • 2
  • F. CockerillIII
    • 1
    • 3
  1. 1.Division of Clinical Microbiology, Department of Laboratory MedicineMayo Clinic and FoundationRochesterUSA
  2. 2.Department of Pediatrics and Adolescent MedicineMayo Clinic and FoundationRochesterUSA
  3. 3.Division of Infectious Diseases, Department of Internal MedicineMayo Clinic and FoundationRochesterUSA

Personalised recommendations