Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Culture ofHelicobacter pylori from gastric biopsies transported in biopsy urease test tubes


Gastric biopsy specimens of 57 consecutively observed dyspeptic patients were studied for the presence ofHelicobacter pylori by histological examination, biopsy urease test (BUT) and culture. For culture, biopsy samples were transported in both Stuart media and BUT tubes. All 15 isolates could be cultured from both Stuart and BUT tubes. Thus, if the main reason for culture ofHelicobacter pylori is for antimicrobial susceptibility testing, only positive BUT tubes need to be submitted. This would reduce both the expense and the number of biopsies needed.

This is a preview of subscription content, log in to check access.


  1. 1.

    Goodwin CS, Blincow ED, Warren JR, Waters TE, Sanderson CR, Easton L: Evaluation of cultural techniques for isolatingCampylobacterpyloridis from endoscopie biopsies of gastric mucosa. Journal of Clinical Pathology 1985, 38: 1127–1131.

  2. 2.

    Cayla R, Zerbib F, Talbi P, Mégraud F, Lamouliatte H: Pre- and post-treatment clarithromycin resistance ofHelicobacter pylori strains: a key factor of treatment failure. Gut 1995, 37, Supplement 1: A55.

  3. 3.

    Rautelin H, Seppälä K, Renkonen OV, Vainio U, Konsunen TU: Role of metronidazole resistance in therapy ofHelicobacter pylori infections. Antimicrobial Agents and Chemotherapy 1992, 36: 163–166.

  4. 4.

    Xia HX, Buckley M, Hyde D, Keane CT, O'Morain CA: Effects of antibiotic resistance on clarithromycin-combined triple therapy forHelicobacter pylori. Gut 1995, 37, Supplement 1: A55.

  5. 5.

    Veenendaal RA, Lichtendahl-Bernards AT, Peña AS, Endtz HP, van Boven CPA, Lamers CBHW: Effect of transport medium and transportation time on culture ofHelicobacter pylori from gastric biopsy specimens. Journal of Clinical Pathology 1993, 46: 561–563.

  6. 6.

    Soltesz V, Zeeberg B, Wadström T: Optimal survival ofHelicobacter pylori under various transport conditions. Journal of Clinical Microbiology 1992, 30: 1453–1456.

  7. 7.

    Xia HX, Keane CT, Chen J, Zhang JJ, Walsh EJ, Moran AP, Hua JS, Mégraud F, O'Morain CA: Transportation ofHelicobacter pylori cultures by optimal systems. Journal of Clinical Microbiology 1994, 32: 3075–3077.

  8. 8.

    Han SW, Flamm R, Hachem CY, Kim HY, Clarridge JE, Evans DG, Beyer J, Drnec J, Graham DY: Transport and storage ofHelicobacter pylori from gastric mucosal biopsies and clinical isolates. European Journal of Clinical Microbiology & Infectious Diseases 1995, 14: 349–352.

  9. 9.

    Cutler AF, Havstad S, Ma CK, Blaser MJ, Perez-Perez Gl, Schubert TT: Accuracy of invasive and noninvasive tests to diagnoseHelicobacter pylori infection. Gastroenterology 1995, 109: 136–141.

  10. 10.

    Marshall BJ, Warren JR, Francis GJ, Langton SR, Goodwin CS, Blincow ED: Rapid urease test in the management ofCampylobacter pyloridis-associated gastritis. American Journal of Gastroenterology 1987, 82: 200–210.

  11. 11.

    Xia HX, Keane CT, O'Morain CA: Pre-formed urease activity ofHelicobacter pylori as determined by a viable count technique — clinical implications. Journal of Medical Microbiology 1994, 40: 435–439.

  12. 12.

    Mobley HLT, Cortesia MJ, Rosenthal LE, Jones BD: Characterization of urease fromCampylobacter pylori. Journal of Clinical Microbiology 1988, 26: 831–836.

  13. 13.

    Marshall BJ, Barrett LJ, Prakash C, McCallum RW, fGuerrant RL: Urea protectsHelicobacter (Campylobacter) pylori from the bactericidal effect of acid. Gastroenterology 1990, 99: 697–702.

Download references

Author information

Correspondence to H. Rautelin.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rautelin, H., Seppälä, K., Nuutinen, H. et al. Culture ofHelicobacter pylori from gastric biopsies transported in biopsy urease test tubes. Eur. J. Clin. Microbiol. Infect. Dis. 16, 380–383 (1997).

Download citation


  • Internal Medicine
  • Histological Examination
  • Test Tube
  • Biopsy Specimen
  • Susceptibility Testing