Phase contrast microscopy affords a rapid and easy way of counting chromosomes from root-tip cells of Angiosperms. The staining method is a modified Feulgen process, the chromosomes being understained before examination under phase. This cuts down even further the time for obtaining satisfactory chromosome counts. Under phase and using a green filter the understained chromosomes in squashes, after only 30 minutes in Feulgen, appear jet black within an almost transparent cell. There is no interference from the cellulose cell walls.
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Haskell, G., Paterson, E.B. The phase contrast microscope in plant cytology. Genetica 33, 52–58 (1963). https://doi.org/10.1007/BF01725747
- Cell Wall
- Phase Contrast
- Phase Contrast Microscope
- Staining Method