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The phase contrast microscope in plant cytology

Abstract

Phase contrast microscopy affords a rapid and easy way of counting chromosomes from root-tip cells of Angiosperms. The staining method is a modified Feulgen process, the chromosomes being understained before examination under phase. This cuts down even further the time for obtaining satisfactory chromosome counts. Under phase and using a green filter the understained chromosomes in squashes, after only 30 minutes in Feulgen, appear jet black within an almost transparent cell. There is no interference from the cellulose cell walls.

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References

  1. Bajer, A. (1957). Ciné-micrographic studies on mitotis in endosperm. 3. The origin of the mitotic spindle.Exptl. Cell Res. 13: 493–502.

  2. Britton, D. M. &J. W. Hull (1956). Mitotic instability in blackberry seedlings.J. Hered. 47: 205–210.

  3. Green, J. A. (1957). Luxol fast scarlet C as a stain for phase-contrast microscope.Stain Technol. 32: 196–197.

  4. Haskell, G. &N. N. Tun (1961). Developmental sequence of chromosome number in a cytologically unstableRubus hybrid.Genet. Res. 2: 10–24.

  5. LaCour, L. F. (1949). InProc. Linnean Soc., London,161, p. 127.

  6. Tun, N. N. &G. Haskell (1961). A squash technique for the small chromosomes ofRubus andRibes.Hort. Res.,1: 62–63.

  7. Wood, M. L. &J. A. Green (1958). Luxol Brilliant green BL as a nuclear stain for phase-contrast microscopy.Stain Technol. 33: 301–302.

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Haskell, G., Paterson, E.B. The phase contrast microscope in plant cytology. Genetica 33, 52–58 (1963). https://doi.org/10.1007/BF01725747

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Keywords

  • Cellulose
  • Cell Wall
  • Phase Contrast
  • Phase Contrast Microscope
  • Staining Method