For monitoring retroviral infection on the gene level, we propose the use of quantitative PCR with two internal standards: one for a fragment of the viral genome and the other for the host cell marker gene. The standards (one for HIV and the other for a human DNA marker gene HLA-DQα) were constructed by PCR-mediated joining of DNA fragments and were found to be effective in quantitative PCR despite rather different structures of amplified fragments in target and standard DNAs. The number of HIV DNA copies was found to be 2–500 per 1000 lymphocytes in blood from HIV-infected patients and up to 5000+ per 1000 cells in chronically infected cell lines. The degree of infection thus measured was found to change over the course of treatment.
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Kellog S.E., Sninsky J.J., and Kwok S., Analyt Biochem189 202–208, 1990.
Diviacco S., Norio P., Zentilin L., Menzo C., Clementi M., Biamonti G., Riva C., Falaschi A., and Giacca M., Gene122 313–320, 1992.
Kohsaka H., Taniguchi A., Richmann D.D., and Carson D.A., Nucleic Acids Res21 3469–3472, 1993.
Pannetier C., Delassus S., Darche S., Saucire C., and Kourilsky P., Nucleic Acids Res21 577–583, 1993.
Bush C.E., Donovan R.M., Smereck S.M., Strang D., Markowitz N., and Saravolatz L.D. AIDS Res Hum Retroviruses9 183–187, 1993.
Yolov A.A., Kozlova A.V., Mednikov B.M., Kornilayeva G.V., Papuashvili M.N., Prokopenko V.D., and Karamov E.V., Voprosy Virusologii39 107–110, 1994.
Yolov A.A. and Shabarova Z.A., Nucleic Acids Res18 3983–3986, 1990.
Smelkova N.V., Yolo A.A., and Shabarova Z.A., Bioorgan Khimiya18 78–84, 1992.
Bianchi N., Mischiati C., Ferioto G., and Gambari R., Nucleic Acids Res21 3595–3596, 1993.
Becker-Andre M. and Hahlbrock K., Nucleic Acids Res17 9437–9446, 1989.
Horton R.M., Hunt H.D., Ho S.N., Pullen J.K., and Pease L.R., Gene77 61–68, 1989.
Gyllensten U.F. and Ehrlich H.A., Proc Nat Acad Sci USA85 7652–7656, 1988.
D'Aquilla R.T., Bechtel L.J., Videler J.A., Eron J.J., Gorczyla P., and Kaplan J.C., Nucleic Acids Res19 749, 1991; see also Perkin Elmer Biotechnology Catalog, 1992–1993, pp. 14–15, 61.
Scharf S.J., Horn G.T., and Ehrlich H.A., Science233 1076–1078, 1986.
Bagasra O., Seshamma T., Oakes J.W., and Pomerantz R.J.P., AIDS7(Suppl 2), S7-S10, 1993.
Bruisten S.M., Koppelman M.H.G.M., Roos M.T.L., Loeliger A.E., Reiss P., Boucher C.A.B., and Huisman H.G., AIDS,7(Suppl 2), S15-S20, 1993.
Karamov E.V. and Lukashov V.V., Molekuliarnaya Biologiya28 5–18, 1994.
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Yolov, A.A., Kozlova, A.V., Yaroslavtseva, N.G. et al. Quantitative PCR as a method for monitoring retroviral infection on the gene level. Virus Genes 10, 45–51 (1995). https://doi.org/10.1007/BF01724296
- retroviral infection
- quantitative PCR
- AIDS therapy
- recombinant DNA