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Chemiluminescence assay for detection of anti-platelet antibodies

Chemilumineszenztest zum Nachweis von Thrombozyten-Antikörpern

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Summary

A chemiluminescence (CL) assay for detection of antiplatelet antibodies was developed. Platelets sensitized with a xenogeneic antiplatelet serum or a potent anti-P1A1 serum stimulated granulocytes to produce CL. Other antibodies detectable by51chromium release and immunofluorescent assays were not discerned by the CL assay.

Zusammenfassung

Es wird über einen neu entseidselten Chemilumineszenztest zum Nachweis von Thrombozyten Antikörpern berichtet. Mit einem tierischen Antiserum gegen Thrombozyten oder einem starken Anti-P1A1 beladene Thrombozyten regen Granulozyten zur Chemilumineszenz an. Andere Antikörper, die durch Chrom51 Freisetzung oder mit Immunfluoreszenztest erkennbar sind, sind im Chemilumineszenztest nicht erkennbar.

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Correspondence to J. E. Menitove MD.

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Menitove, J.E., Frenzke, M.E., Braem, S.W. et al. Chemiluminescence assay for detection of anti-platelet antibodies. Klin Wochenschr 60, 567–569 (1982). https://doi.org/10.1007/BF01724213

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Key words

  • Chemiluminescence
  • Antiplatelet antibodies

Schlüsselwörter

  • Chemilumineszenz
  • Thrombozyten Antikörpern