Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Anti-thoracic duct lymphocyte globulin stimulates human megakaryocytopoiesis in vitro

  • 20 Accesses

  • 3 Citations


Anti-thoracic duct lymphocyte globulin (ALG) therapy is effective in patients with aplastic anemia. We examined the effect of ALG on human megakaryocyte progenitor cells (colony-forming unit-megakaryocyte, CFU-Meg) in vitro. Normal human bone marrow mononuclear cells (MNC) were cultured in plasma clots with varying concentrations of ALG or non-immunized horse IgG. After 12 days of culture, significant megakaryocyte colony formation was observed in cultures containing ALG but not in cultures containing non-immunized horse IgG. The peak stimulatory effect seemed to occur with 10–25µg/ml of ALG. When marrow MNC, depleted of adherent and T cells, were cultured in plasma clots with ALG, its stimulatory effect on megakaryocytopoiesis decreased markedly. Finally, it was demonstrated that ALG stimulated marrow MNC to produce a factor stimulatory for CFU-Meg. The in vitro megakaryocytopoietic stimulatory effect of ALG may be related to its clinical efficacy in some patients with aplastic anemia.

This is a preview of subscription content, log in to check access.


  1. 1.

    Ascensao JA, Pahwa R, Kagan W, Hansen J, Moore M, Good R (1976) Aplastic anaemia: evidence for an immunological mechanism. Lancet I: 669–671

  2. 2.

    Camitta BM, Storb R, Thomas ED (1982) Aplastic anemia. N Engl J Med 306: 645–652

  3. 3.

    Champlin R, Ho W, Gale RP (1983) Antithymocyte globulin treatment in patients with aplastic anemia. A prospective randomized trial. N Engl J Med 308: 113–118

  4. 4.

    Faille A, Barrett AJ, Balitrand N, Ketels F, Gluckman E, Najean Y (1979) Effect of antilymphocyte globulin on granulocyte precursors in aplastic anaemia. Br J Haematol 42: 371–380

  5. 5.

    Gascon P, Zoumbos NC, Scala G, Djeu JY, Moore JG, Young NS (1985) Lymphokine abnormalities in aplastic anemia: implications for the mechanism of action of antithymocyte globulin. Blood 65: 407–413

  6. 6.

    Gluckman E, Devergie A, Faille A, Barrett AJ, Bonneau M, Boiron M, Bernard J (1978) Treatment of severe aplastic anemia with antilymphocyte globulin and androgens. Exp Hematol 6: 679–687

  7. 7.

    Gmelig-Meyling F, Ballieux R (1977) Simplified procedure for the separation of human T and non-T cells. Vox Sang 33: 5–8

  8. 8.

    Hoffman R, Mazur E, Bruno E, Floyd V (1981) Assay of an activity in the serum of patients with disorders of thrombopoiesis that stimulates formation of megakaryocyte colonies. N Engl J Med 305: 533–538

  9. 9.

    Hsu S-M, Raine L, Fanger H (1981) The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics. Am J Clin Pathol 75: 816–821

  10. 10.

    Huang AT, Mold NG, Zhang S-F (1987) Antithymocyte globulin stimulates human hematopoietic progenitor cells. Proc Natl Acad Sci USA 84: 5942–5946

  11. 11.

    Jansen J, Veenhof WFJ, Goselink HM (1981) Anti-thymocyte globulin effective in the treatment of aplastic anemia does not stimulate granulocyte precursor cells. Exp Hematol 9: 1028–1034

  12. 12.

    Kawano Y, Nissen C, Gratwohl A, Speck B (1988) Immunostimulatory effects of different antilymphocyte globulin preparations: a possible clue to their clinical effect. Br J Haematol 68: 115–119

  13. 13.

    Messner HA, Till JE, McCulloch EA (1973) Interacting cell populations affecting granulopoietic colony formation by normal and leukemia marrow cells. Blood 42: 701–710

  14. 14.

    Netzel B, Rodt H, Hoffmann-Fezer G, Thiel E, Thierfelder S (1978) The effect of crude and differently absorbed antihuman T-cell globulin on granulocytic and erythropoietic colony formation. Exp Hematol 6: 410–420

  15. 15.

    Ogata K, Kuriya S, Dan K, Nomura T (1989) Partial purification of human urinary megakaryocyte colony-stimulating factor. Exp Cell Biol 57: 19–26

  16. 16.

    Speck B, Gluckman E, Haak HL, Van Rood JJ (1977) Treatment of aplastic anaemia by antilymphocyte globulin with and without allogeneic bone marrow infusions. Lancet II: 1145–1148

  17. 17.

    Wu AM (1979) Properties and separation of T lymphocyte growth stimulatory activity (TL-CSA) and of granulocytemacrophage colony stimulating activity (GM-CSA) produced separately from two human T lymphocyte subpopulations. J Cell Physiol 101: 237–250

Download references

Author information

Correspondence to K. Ogata.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ogata, K., Dan, K., Kuriya, S. et al. Anti-thoracic duct lymphocyte globulin stimulates human megakaryocytopoiesis in vitro. Blut 60, 202–205 (1990). https://doi.org/10.1007/BF01720277

Download citation

Key words

  • Anti-thoracic duct lymphocyte globulin
  • Megakaryocytopoiesis
  • Colony-forming unitmegakaryocyte