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Immunogenetics

, Volume 33, Issue 3, pp 163–170 | Cite as

Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNA

  • Teodorica L. Bugawan
  • Henry A. Erlich
Original articles

Abstract

The allelic sequence diversity at theHLA-DQBI locus has been analyzed by polymerase chain reaction (PCR) amplification and sequencing. Fifteen amino acid sequence-defined alleles (one previously unreported) and several silent nucleotide polymorphisms which subdivide these alleles have been identified. Here, we describe the specific amplification of theDQB1 second exon by several different PCR primer pairs and a simple and rapid typing procedure using a panel of 16 horseradish peroxidase (HRP)-labeled oligonucleotide probes capable of distinguishing theseDQBI alleles.

Keywords

Polymerase Chain Reaction Nucleotide Prime Pair Sequence Diversity Horseradish Peroxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Teodorica L. Bugawan
    • 1
  • Henry A. Erlich
    • 1
  1. 1.Department of Human GeneticsCetus CorporationEmeryvilleUSA

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