Archives of Virology

, Volume 141, Issue 12, pp 2365–2374 | Cite as

Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis

  • J. P. G. Leite
  • A. A. Alfieri
  • P. A. Woods
  • R. I. Glass
  • J. R. Gentsch
Original Papers


We used reverse transcription-polymerase chain reaction (RT-PCR) to determine the P and G genotypes of 130 culture-adapted rotavirus strains isolated from 181 fecal specimens of children under 5 years of age from 9 states and the Federal District of Brazil. The 4 genotypes found most commonly worldwide were also common in Brazil and P[8]G1 was the most prevalent (43%), followed by P[4]G2 (12%), P[8]G3 (6%), and P[8]G4 (6%). However, unusual types P[8]G5, P[6]G2, P[9]G1, P[9]G3, and mixed infections were responsible for 12% and 21% of the cases, respectively. Genotype G5 strains were detected in specimens collected in all 9 areas surveyed from all 4 regions of Brazil. The unusual strain diversity in Brazil suggests that when tetravalent rotavirus vaccines currently being developed are introduced into Brazil, laboratory surveillance will be essential to monitor protection against unusual strains, particularly those of genotype 5, as well as emergence of novel reassortants that may evolve from the large pool of children with mixed infections.


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. P. G. Leite
    • 1
    • 3
  • A. A. Alfieri
    • 1
    • 2
  • P. A. Woods
    • 3
  • R. I. Glass
    • 3
  • J. R. Gentsch
    • 3
  1. 1.Department of VirologyOswaldo Cruz InstituteRio de JaneiroBrazil
  2. 2.Laboratory of Animal VirologyLondrina State UniversityParanaBrazil
  3. 3.Viral Gastroenteritis Section, Division of Viral and Rickettsial DiseasesNational Center for Infectious Diseases, Centers for Disease Control and PreventionAtlantaUSA

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