Archives of Virology

, Volume 141, Issue 1, pp 1–12 | Cite as

Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains

  • K. Kojima
  • K. Taniguchi
  • N. Kobayashi
Original Papers


We have sequenced gene 5 encoding NSP1 for three human, two porcine, two bovine, one feline, and five equine rotavirus strains, and compared the nucleotide and deduced amino acid sequences with the published sequences for other various strains. Subgroup I human strains L26, 69M, and DS-1 were found to have a similar NSP1 sequence despite their different G serotypes, VP4 genotypes, and RNA patterns. The NSP1 sequence of the human strain K8 showed a high degree of homology to those of porcine strains OSU and YM. A high degree of homology was found among three equine strains (H2, FI-14, and FI23), but they differed from the other equine strains L338 and H1. The strain H1 was similar to the porcine strains. The feline strain Cat2 showed a high homology to bovine strains UK, RF, and A44. Thus, species-specific and interspecies relatedness of NSP1 sequences among human, porcine, bovine, feline and equine rotaviruses was found. Overall genomic relatedness of strains L26 and YM to various human and animal strains was also examined by RNA-RNA hybridization assay. The present and previous hybridization results showed that there is a good correlation in most strains between overall genomic property (or genogroup) and NSP1 sequence homology.


Deduce Amino Acid Sequence Genomic Relatedness Human Strain Animal Strain Porcine Strain 
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  1. 1.
    Both GW, Bellamy AR, Mitchell DB (1994) Rotavirus protein structure and function. Curr Top Microbiol Immunol 185: 67–105Google Scholar
  2. 2.
    Bremont M, Charpilienne A, Chabanne D, Cohen J (1987) Nucleotide sequence and expression inEscherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. Virology 161: 138–144Google Scholar
  3. 3.
    Brottier P, Nandi P, Bremont M, Cohen J (1992) Bovine rotavirus segment 5 protein expressed in the baculovirus system interacts with zinc and RNA. J Gen Virol 73: 1931–1938Google Scholar
  4. 4.
    Dunn SJ, Cross TL, Greenberg HB (1994) Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and Northern blot hybridization. Virology 203: 178–183Google Scholar
  5. 5.
    Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449Google Scholar
  6. 6.
    Flores J, Perez I, White L, Perez M, Kalica AR, Marquina R, Wyatt RG, Kapikian AZ, Chanock RM (1982) Genetic relatedness among human rotaviruses as determined by RNA hybridization. Infect Immun 37: 648–655Google Scholar
  7. 7.
    Gombold JL, Ramig RF (1986) Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV. J Virol 57: 110–116Google Scholar
  8. 8.
    Gorziglia M, Hoshino Y, Buckler-White A, Blumentals I, Glass R, Flores J, Kapikian AZ, Chanock RM (1986) Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci USA 83: 7039–7043Google Scholar
  9. 9.
    Graham A, Kudesia G, Allen AM, Desselberger U (1987) Reassortment of human rotavirus possessing genome rearrangements with bovine rotavirus: evidence for host cell selection. J Gen Virol 68: 115–122Google Scholar
  10. 10.
    Hein J (1990) Unified approach to alignment and phylogenies. Methods Enzymol 183: 626–645Google Scholar
  11. 11.
    Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci 8: 189–191Google Scholar
  12. 12.
    Hua J, Chen X, Patton JT (1994) Deletion mapping of the rotavirus metalloprotein NS53 (NSP1): the conserved cysteine-rich region is essential for virus-specific RNA binding. J Virol 68: 3990–4000Google Scholar
  13. 13.
    Hua J, Mansell EA, Patton JT (1993) Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA. Virology 196: 372–378Google Scholar
  14. 14.
    Hua J, Patton JT (1994) The carboxyl-half of the rotavirus nonstructural protein NS53 (NSP1) is not required for virus replication. Virology 198: 567–576Google Scholar
  15. 15.
    Kapikian AZ, Chanock RM (1990) Rotaviruses. In: Fields BN, Knipe DM, Chanock RM, Hirsch MS, Melnick JL, Monath TP, Roizman B (eds) Virology, 2nd ed. Raven Press, New York, pp 1353–1404Google Scholar
  16. 16.
    Kobayashi N, Kojima K, Taniguchi K, Urasawa T, Urasawa S (1994) Genotypic diversity of reassortants between simian rotavirus SA11 and human rotaviruses having different antigenic specificities and RNA patterns. Res Virol 145: 303–311Google Scholar
  17. 17.
    Kobayashi N, Taniguchi K, Urasawa T, Urasawa S (1994) Effect of the selection pressure with anti-VP7 and anti-VP4 neutralizing monoclonal antibodies on reassortant formation between two human rotaviruses. Arch Virol 135: 383–396Google Scholar
  18. 18.
    Mitchell DB, Both GW (1990) Conservation of a potential metal binding motif despite extensive sequence diversity in the rotavirus nonstructural protein NS53. Virology 174: 618–621Google Scholar
  19. 19.
    Myers EW, Miller W (1988) Optimal alignments in linear space. Comput Appl Biosci 4: 11–17Google Scholar
  20. 20.
    Nakagomi O, Kaga E, Nakagomi T (1992) Human rotavirus strain with unique VP4 neutralization epitopes as a result of natural reassortment between members of the AU-1 and Wa genogroups. Arch Virol 127: 365–371Google Scholar
  21. 21.
    Nakagomi O, Nakagomi T (1991) Genetic diversity and similarity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. Arch Virol 120: 43–55Google Scholar
  22. 22.
    Nakagomi O, Ohshima A, Aboudy Y, Shif I, Mochizuki M, Nakagomi T, Gotlieb-Stematsky T (1990) Molecular identification by RNA-RNA hybridization of a human rotavirus that is closely related to rotaviruses of feline and canine origin. J Clin Microbiol 28: 1198–1203Google Scholar
  23. 23.
    Nishikawa K, Hoshino Y, Taniguchi K, Green KY, Greenberg HB, Kapikian AZ, Chanock RM, Gorziglia M (1989) Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171: 503–515Google Scholar
  24. 24.
    Ohshima A, Takagi T, Nakagomi T, Matsuno S, Nakagomi O (1990) Molecular characterization by RNA-RNA hybridization of a serotype 8 human rotavirus with ‘super-short’ RNA electropherotype. J Med Virol 30: 107–112Google Scholar
  25. 25.
    Palombo EA, Bishop RF (1994) Genetic analysis of NSP1 genes of human rotaviruses isolated from neonates with asymptomatic infection. J Gen Virol 75: 3635–3639Google Scholar
  26. 26.
    Patton JT, Salter-Cid L, Kalbach A, Mansell EA, Kattoura M (1993) Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35. Virology 192: 438–446Google Scholar
  27. 27.
    Ramig RF, Ward RL (1991) Genomic segment reassortment in rotaviruses and other reoviridae. Adv Virus Res 39: 163–207Google Scholar
  28. 28.
    Sereno MM, Gorziglia MI (1994) The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type. Virology 199: 500–504Google Scholar
  29. 29.
    Taniguchi K, Nishikawa K, Urasawa T, Urasawa S, Midthun K, Kapikian AZ, Gorziglia M (1989) Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. J Virol 63: 4101–4106Google Scholar
  30. 30.
    Taniguchi K, Urasawa T, Kobayashi N, Gorziglia M, Urasawa S (1990) Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol 64: 5640–5644Google Scholar
  31. 31.
    Taniguchi K, Urasawa T, Pongsuwanna Y, Choonthanom M, Jayavasu C, Urasawa S (1991) Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol 72: 2929–2937Google Scholar
  32. 32.
    Taniguchi K, Urasawa T, Urasawa S (1994) Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology 200: 390–400Google Scholar
  33. 33.
    Taniguchi K, Wakasugi F, Pongusuwanna Y, Urasawa T, Ukae S, Chiba S, Urasawa S (1992) Identification of human and bovine rotavirus serotypes by polymerase chain reaction. Epidemiol Infect 109: 303–312Google Scholar
  34. 34.
    Tian Y, Tarlow O, Ballard A, Desselberger U, McCrae MA (1994) Genomic concatemerization/deletion in rotaviruses: a new mechanism for generating rapid genetic change of potential epidemiological importance. J Virol 67: 6625–6632Google Scholar
  35. 35.
    Urasawa S, Hasegawa A, Urasawa T, Taniguchi K, Wakasugi F, Suzuki H, Inouye S, Pongprot B, Supawadee J, Suprasert S, Rangsiyanond P, Tonusin S, Yamazi Y (1992) Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses. J Infect Dis 166: 227–234Google Scholar
  36. 36.
    Urasawa S, Urasawa T, Wakasugi F, Kobayashi N, Taniguchi K, Lintag IC, Saniel MC, Goto H (1990) Presumptive seventh serotype of human rotavirus. Arch Virol 113: 279–282Google Scholar
  37. 37.
    Ward RL, Nakagomi O, Knowlton DR, McNeal MM, Nakagomi T, Clemens JD, Sack DA, Schiff GM (1990) Evidence for natural reassortants of human rotaviruses belonging to different genogroups. J Virol 64: 3219–3225Google Scholar
  38. 38.
    Wu H, Taniguchi K, Urasawa T, Urasawa S (1993) Genomic relatedness of five equine rotavirus strains with different G serotype and P type specificities. Res Virol 144: 455–464Google Scholar
  39. 39.
    Xu L, Tian Y, Tarlow O, Harbour D, McCrae MA (1994) Molecular biology of rotaviruses. IX. Conservation and divergence in genome segment 5. J Gen Virol 75: 3413–3421Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Kojima
    • 1
  • K. Taniguchi
    • 1
  • N. Kobayashi
    • 1
  1. 1.Department of HygieneSapporo Medical University School of MedicineSapporoJapan

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