Archives of Virology

, Volume 141, Issue 2, pp 381–390 | Cite as

Sequence conservation and expression of the gene encoding the outer capsid glycoprotein among human group C rotaviruses of global distribution

  • B. Jiang
  • H. Tsunemitsu
  • P. H. Dennehy
  • I. Oishi
  • D. Brown
  • R. D. Schnagl
  • M. Oseto
  • Z. -Y. Fang
  • L. F. Avendano
  • L. J. Saif
  • R. I. Glass
Brief Report

Summary

Group C rotaviruses have been identified recently from fecal samples of children with diarrhea in the United States. Using reverse transcriptase-polymerase chain reaction and sequence analysis, we sequenced gene 8s encoding VP7 from two U.S. strains (RI-1 and RI-2), and eight other strains isolated from patients on four continents, and compared these with the sequences of four published strains. The gene 8s of the 14 strains were remarkably conserved in size and in predicted primary and secondary structures. When the sequences of the human VP7s were compared with that of the prototype porcine Cowden strain, six regions were found variable in both deduced primary and predicted secondary structures, four of which were predicted to be hydrophilic and might determine serotype specificity. Gene 8 of the human S-1 strain was further characterized by expression in recombinant baculoviruses. The expressed product was immunogenic but failed to elicit neutralizing antibodies. Our sequence analysis indicates that all the human strains characterized to date belong to a single G genotype, which may constitute a single G serotype, pending further antigenic analysis. Whether the human strains and the Cowden strain are the same serotype remains to be determined.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • B. Jiang
    • 1
  • H. Tsunemitsu
    • 2
  • P. H. Dennehy
    • 3
  • I. Oishi
    • 4
  • D. Brown
    • 5
  • R. D. Schnagl
    • 6
  • M. Oseto
    • 7
  • Z. -Y. Fang
    • 8
  • L. F. Avendano
    • 9
  • L. J. Saif
    • 2
  • R. I. Glass
    • 1
  1. 1.U. S. Department of Health and Human ServicesViral Gastroenteritis Section, Centers for Disease Control and Prevention, Public Health ServiceAtlanta
  2. 2.Food Animal Health Research ProgramOhio State UniversityWooster
  3. 3.Department of PediatricsBrown University School of MedicineProvidence
  4. 4.Osaka Prefectural Institute of Public HealthOsakaJapan
  5. 5.Central Public Health LaboratoryLondonUK
  6. 6.School of MicrobiologyLa Trobe UniversityVictoriaAustralia
  7. 7.Ehime Prefectural Institute of Public HealthEhimeJapan
  8. 8.Institute of VirologyChinese Academy of Preventive MedicineBeijingChina
  9. 9.Laboratorio de VirologiaUniversidad de ChileSantiagoChile

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