Archives of Virology

, Volume 141, Issue 3–4, pp 635–647 | Cite as

Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” based on the nucleotide sequence of the fusion protein gene

  • M. S. Collins
  • I. Strong
  • D. J. Alexander
Original Papers


The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an intravenous pathogenicity index in chickens (IVPI) of 2.01 and isolate 1168/84 which had received six passages in chickens and had an IVPI of 0.00. The F genes of virus from two passage levels of isolate 1447/84, 0 with IVPI value 0.00 and six with IVPI value 0.58, were partially sequenced to cover the areas of variation between 760/83 and 1168/84. The two passage levels of 1447/84 showed identical sequences in these areas which in turn were identical to those of 760/83. It was concluded that the recorded differences in intravenous pathogenicity were unlikely to be associated with differences in the primary structure of the F0 protein. Phylogenetic comparisons of the F gene sequences of the two PPMV-1 viruses with those published for other NDV strains and isolates showed that the PPMV-1 viruses formed a new fourth lineage but were closely related to strain Warwick with which they presumably shared a common origin.


Infectious Disease Amino Acid Sequence Nucleotide Sequence Gene Sequence Fusion Protein 
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. S. Collins
    • 1
  • I. Strong
    • 1
  • D. J. Alexander
    • 1
  1. 1.Virology DepartmentCentral Veterinary Laboratory (Weybridge)AddlestoneU.K.

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