Archives of Virology

, Volume 141, Issue 3–4, pp 439–447 | Cite as

Heat shock induces HIV-1 replication in chronically infected promyelocyte cell line OM10.1

  • K. Hashimoto
  • M. Baba
  • K. Gohnai
  • M. Sato
  • S. Shigeta
Original Papers

Summary

A long period of clinical latency before development of symptoms is characteristic of human immunodeficiency virus type 1 (HIV-1) infection. OM10.1, a promyelocyte cell line latently infected with HIV-1, has been developed as a model for studying the mechanism of viral latency and the activation of virus expression. We found that this latently infected cell line with heat shock at 42°C for 2 h resulted in a high level of HIV-1 production without addition of any cytokines. The mechanism of activation was analyzed by using anti-TNF-α antibody and various inhibitors. Although the TNF-α level in culture supernatants was below the sensitivity of an ELISA assay system, addition of anti-TNF-α antibody in culture medium could partially suppress the heat shock induced HIV-1 production. Staurosporine (PKC inhibitor), pentoxifylline (NF-ϰB inhibitor), and Ro5-3335 (HIV-1 Tat inhibitor) also inhibited significantly the heat shock induced virus activation. In particular, staurosporine achieved approximately 90% inhibition of the HIV-1 antigen expression in heat shock-treated OM10.1 at a non-toxic concentration. Although the mechanism of HIV-1 activation with heat shock has not been fully elucidated yet, it is presumed PKC plays an important role in HIV-1 activation. Thus, the present observations will provide a further insight into the pathogenesis of HIV-1 infections.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Hashimoto
    • 1
  • M. Baba
    • 2
  • K. Gohnai
    • 3
  • M. Sato
    • 1
  • S. Shigeta
    • 1
  1. 1.Department of MicrobiologyFukushima Medical CollegeFukushimaJapan
  2. 2.Division of Human Retroviruses, Center for Chronic Viral Diseases, Faculty of MedicineKagoshima UniversityKagoshimaJapan
  3. 3.Roku-chome ClinicSendaiJapan

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