Ten presumptive enterovirus isolates which could not be neutralized by type specific antisera to any prototype enterovirus were related to echovirus 22 using molecular, biologic and serologic methods. Viral protein fingerprinting and PCR first suggested that these strains were variants of echovirus 22. Three of the strains were echovirus 22 prime strains, i.e., antiserum made to the variant strain neutralized the variant and the prototype strain. The other strains were neutralized by antiserum to the prime strains. Unlike typical enteroviruses, echovirus 22 and 23 prototype viruses and 7 of the 10 variants were heat stable at 50 °C in H2O for 1 h.
KeywordsInfectious Disease Viral Protein Variant Strain Specific Antiserum Serologic Method
Unable to display preview. Download preview PDF.
- 4.Ehrnst A, Eriksson M (1993) Epidemiological features of type 22 echovirus infection. Scand J Infect Dis 25: 278–281Google Scholar
- 5.Grandien M, Forsgren M, Ehrnst A (1989) Enteroviruses and reoviruses. In: Schmidt NJ, Emmons RW (eds) Diagnostic procedures for viral, rickettsial and chlamydial infections, 6th ed. American Public Health Association, Washington, p 521Google Scholar
- 6.Gwaltney JM, Colonno RJ, Hamparian VV, Turner RB (1989) Rhinoviruses. In: Schmidt NJ, Emmons RW (eds) Diagnostic procedures for viral, rickettsial and chlamydial infections, 6th ed. American Public Health Association, Washington, pp 602–603Google Scholar
- 12.Shimizu C, Rambaud C, Cheron G, Rouzioux C, Lozinski GM, Rao A, Stanway G, Krous HF, Burns JC (1996) Molecular identification of viruses in sudden infant death associated with myocarditis and pericarditis. Ped Infect Dis (in press)Google Scholar
- 15.Walpita P, Connor JD, Pfeifer D (1988) Protein fingerprinting: a novel virus identification system. J Virol Methods 24: 315–324Google Scholar