Archives of Virology

, Volume 141, Issue 8, pp 1423–1436

Oral immunization of rabbits with VP60 particles confers protection against rabbit hemorrhagic disease

  • J. Plana-Duran
  • M. Bastons
  • M. J. Rodriguez
  • I. Climent
  • E. Cortés
  • C. Vela
  • I. Casal
Original Papers

Summary

Rabbit hemorrhagic disease virus (RHDV) causes more than 90% mortality in adult rabbits. In this study, the cDNA of the VP60 coding sequence of RHDV was cloned under the control of the polyhedrin and p10 promoters of baculovirus to be expressed in insect cells. The expression of RHDV VP60 under the control of the p10 promoter was 5–10 times higher than using the polyhedrin promoter. The p10-derived VP60 was able to assemble into virus-like particles (VLPs). RHDV VLPs were successfully used to protect rabbits against the disease even at doses as low as 0.5 µg when injected intramuscularly or sub-cutaneously. The ability to elicit an immune response was independent of the adjuvant or the route of immunization. Remarkably, oral administration of RHDV VLPs efficiently induced protecting antibodies to RHD at doses as low as 3 µg. The use of binary ethylenimine for the stabilization of the VLPs was decisive for eliciting a good oral immunity. This report demonstrates the potential use of these procapsids in obtaining RHD oral vaccines and opens the door to the use of these capsids for the prevention of the disease in wild animals. Therefore, a new, and potentially important application of recombinant VLPs in the induction of protective immunity by the oral route is foreseen.

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References

  1. 1.
    Bahnemann HG (1990) Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine. Vaccine 8: 299–303Google Scholar
  2. 2.
    Boga JA, Casais R, Marin MS, Martin-Alonso JM, Carmenes RS, Prieto M, Parra F (1994) Molecular cloning, sequencing and expression in Escherichia coli of the capsid protein gene from rabbit haemorrhagic disease virus (Spanish isolate AST/89). J Gen Virol 75: 2 409–2 413Google Scholar
  3. 3.
    Blancou J, Kieny MP, Lathe R, Lecocq JP, Pastoret PP, Soulebot JP, Desmettre P (1986) Oral vaccination of the fox against rabies using a live recombinant vaccinia virus. Nature 322: 373–375Google Scholar
  4. 4.
    Brochier B, Kieny MP, Costy F, Coppens P, Bauduin B, Curtis PJ, Wuner WH (1991) Large scale eradication of rabies using recombinant vaccinia-rabies vaccine. Nature 354: 520–522Google Scholar
  5. 5.
    Casal JI, Meloen R, Dalsgaard K (1995) Development of second generation vaccines against parvoviruses. In: Vassarotti A, Kalbe-Bournonville L, Baselga de Elorz J, de Taxis du Poet Ph (eds) Bridge. Final report. European Commision, Luxembourg, pp 373–376Google Scholar
  6. 6.
    Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1986) Lipofection. A highly efficient, lipid mediated DNA-transfection procedures. Proc Natl Acad Sci USA 84: 7 413–7 417Google Scholar
  7. 7.
    Fu ZF, Rupprecht CE, Dietzschold B, Saikumar P, Niu HS, Babka I, Wunner WH, Koprowski H (1993) Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein. Vaccine 11: 925–928Google Scholar
  8. 8.
    Laurent S, Vautherot J-F, Madelaine M-F, Le Gall G, Rasschaert D (1994) Recombinant rabbit hemorraghic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection. J Virol 68: 6794–6798Google Scholar
  9. 9.
    Liu SJ, Xue HP, Pu BQ, Quian NH (1984) A new viral disease in rabbits. Anim Husb Vet Med 16: 253–255Google Scholar
  10. 10.
    Lopez de Turiso JA, Cortés E, Martinez C, Ruiz de ybanez R, Simarro I, Vela C, Casal I (1992) Recombinant vaccine for canine parvovirus in dogs. J Virol 66: 2 748–2 7537Google Scholar
  11. 11.
    Matsuura Y, Posee RD, Overton HA, Bishop DHL (1987) Baculovirus expression vectors The requirements for high level expression of proteins including glycoproteins. J Gen Virol 88: 1 233–1 250Google Scholar
  12. 12.
    McGhee JR, Mestecky J, Dertzbaugh MT, Eldridge JH, Hirasawa M, Kiyono H (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10: 75–88Google Scholar
  13. 13.
    Meyers G, Wirblich C, Thiel H-J (1991) Rabbit hemorrhagic disease virus: Molecular cloning and nucleotide sequence of a calicivirus genome. Virology 184: 664–676Google Scholar
  14. 14.
    Meyers G, Wirblich C, Thiel H-J (1991) Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology 184: 677–686Google Scholar
  15. 15.
    Ohlinger VF, Haas B, Ahl R, Weiland F (1989) Die infektiöse hämorrhagische Krankheit der Kaninchen — eine durch ein Calicivirus verursachte Tierseuche. Tieraerztl Umsch 44: 284–294Google Scholar
  16. 16.
    Ohlinger VF, Haas B, Meyer G, Weiland F, Thiel H-J (1990) Identification and characterization of the virus causing rabbit hemorrhagic disease. J Virol 64: 3 331–3 336Google Scholar
  17. 17.
    Pages Mante A (1989) Consideraciones técnicas de la sueroterapia y la profilaxis vacunal en la enfermedad hemorrágica virica del conejo. Med Vet 6: 285–291Google Scholar
  18. 18.
    Parra F, Prieto M (1990) Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. J Virol 64: 4013–4015Google Scholar
  19. 19.
    Plana J, Vayreda M, Bastons M, Vilá X (1989) Calicivirus: firme candidato como agente inductor de la enfermedad hemorrágica del conejo. Med Vet 6: 87–88Google Scholar
  20. 20.
    Possee RD, Howard SC (1987) Analysis of the polyhedrin gene promoter of theAutographa californica nuclear polyhedrosis virus. Nucleic Acids Res 15: 10233–10248Google Scholar
  21. 21.
    Rappuoli R (1994) Toxin inactivation and antigen stabilization: two different uses of formaldehyde. Vaccine 12: 579–580Google Scholar
  22. 22.
    Sabin AB, Boulger LR (1973) History of the Sabin attenuated poliovirus oral live vaccine strain. J Biol Stand 1: 115–118Google Scholar
  23. 23.
    Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitor. Proc Natl Acad Sci USA 74: 5 463–5 467Google Scholar
  24. 24.
    Summers MD, Smith GE (1987) A manual of methods for baculovirus vectors and insects cell culture procedures. Texas Agric Exp Stat Bull 1555Google Scholar
  25. 25.
    Vlak JM, Schouten A, Usmany M, Belsham GJ, Klinge-Roode EC, Maule AJ, Van Lent JWM, Zuidema D (1990) Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology 179: 312–320Google Scholar
  26. 26.
    Walker RI (1994) New strategies for using mucosal vaccination to achieve more effective immunization. Vaccine 12: 387–400Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. Plana-Duran
    • 1
  • M. Bastons
    • 1
  • M. J. Rodriguez
    • 2
  • I. Climent
    • 1
  • E. Cortés
    • 2
  • C. Vela
    • 2
  • I. Casal
    • 2
  1. 1.Laboratorios Sobrino-CyanamidVall de Bianya
  2. 2.INGENASAMadridSpain
  3. 3.Department of Molecular BiologyStockholm UniversityStockholmSweden

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