Archives of Virology

, Volume 141, Issue 10, pp 1823–1832 | Cite as

A viroid fromBrunfelsia undulata closely related to theColumnea latent viroid

  • R. L. Spieker
Original Papers


A viroid was isolated from symptomlessBrunfelsia undulata plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viroid was transmitted to tomato by mechanical inoculation. Infected tomato plants developed symptoms smilar to those caused by intermediate strains of potato spindle tuber viroid (PSTVd). Cloning and sequencing revealed that the viroid fromBrunfelsia undulata is closely related to theColumnea latent viroid (CLVd). Therefore, the new viroid sequence variant has been named CLVd-B. TheBrunfelsia viroid CLVd-B consists of 373 nucleotides, 215 G + C, 158 A + U with a GC content of 57.6%. The most stable rod-like secondary structure of this viroid has 80 G:C, 39 A:U and 11 G:U base pairs with a minimum free energy of −157.2 kcal/mol (−657.1 kJ/mol). The sequence similarity of the right terminal domain of CLVd-B and of tomato apical stunt viroid (TASVd) is higher than the sequence similarity of these domains comparing CLVd and TASVd.


Sequence Similarity Free Energy Tomato Plant Minimum Free Energy Terminal Domain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • R. L. Spieker
    • 1
  1. 1.Abteilung ViroidforschungMax-Planck-Institut für BiochemieMartinsriedFederal Republic of Germany

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