Archives of Virology

, Volume 141, Issue 5, pp 877–884 | Cite as

Polymerase chain reaction detection of the hemagglutinin gene from an attenuated measles vaccine strain in the peripheral mononuclear cells of children with autoimmune hepatitis

  • H. Kawashima
  • T. Mori
  • K. Takekuma
  • A. Hoshika
  • M. Hata
  • T. Nakayama
Originals Papers


We examined the measles H gene using reverse transcriptase-polymerase chain reaction in peripheral mononuclear cells obtained from 4 pediatric and 2 adult patients with autoimmune hepatitis and 12 healthy children who had been infected with measles or vaccinated with an attenuated measles vaccine in the past. All patients were positive for the presence of the gene. Only one healthy control, who had been vaccinated two weeks before the study, was positive, while the other 11 controls were negative for the presence of the gene. The restriction enzyme patterns of the products in the pediatric patients were different from those observed in adults. The sequences of amplified products from pediatric patients coincided with the vaccine strain, whereas those from adults were different from the vaccine strain. The sequence of those from one of two adult patients was similar to those of the isolates in 1990 and later. Our results demonstrated that children with autoimmune hepatitis can have persistence of the vaccine strain in vivo for many years after vaccination.


Polymerase Chain Reaction Hepatitis Infectious Disease Adult Patient Restriction Enzyme 
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  1. 1.
    Andjaparidze OG, Chaplygina NM, Bogomolova NN, Koptyaeva IB, Nevryaeva EG, Filimonova RG, Tareeva IE (1989) Detection of measles virus genome in blood leukocytes of patients with certain autoimmune diseases. Arch Virol 105: 287–291Google Scholar
  2. 2.
    Black FL (1988) Persistent measles virus genome in autoimmune chronic active hepatitis: cause or coincidence? Hepatology 8: 186–187Google Scholar
  3. 3.
    Cattaneo R, Schmid A, Spielhofer P, Kaelin K, Baczko K, Meulen V, Pardowitz J, Flanagan S, Rima BT, Ueda ST, Billeter M (1989) Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases. Virology 173: 415–425Google Scholar
  4. 4.
    Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159Google Scholar
  5. 5.
    Dyken PR, Cunningham SC, Ward LC (1989) Changing character of subacute sclerosing panencephalitis in the United States. Pediatr Neurol 5: 339–341Google Scholar
  6. 6.
    Gavish D, Kleinman Y, Abraham M, Chajek-Shaul T (1983) Hepatitis and jaundice associated with measles in young adults. Arch Int Med 143: 674–677Google Scholar
  7. 7.
    Laitinen O, Vaheri A (1974) Very high measles and rubella virus antibody titres associated with hepatitis, systemic lupus erythematosus, and infectious mononucleosis. Lancet i: 194–197Google Scholar
  8. 8.
    Mori T (1994) A simple method for genetic differentiation of the AIK-C vaccine strain from wild strains of measles virus. Biologicals 22: 179–185Google Scholar
  9. 9.
    Mori T, Sasaki K, Hashimoto H, Makino S (1993) Molecular cloning and complete nucleotide sequence of genomic RNA of the AIK-C strain of attenuated measles virus. Virus Genes 7: 67–81Google Scholar
  10. 10.
    Nakayama T, Morti T, Yamaguchi S, Sonoda S, Asamura S, Yamashita R, Takeuchi Y, Urano T (1995) Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability. Virus Res 35: 1–5Google Scholar
  11. 11.
    Robertson DA, Zhang SL, Guy EC, Wright R (1987) Persistent measles virus genome in autoimmune chronic active hepatitis. Lancet ii: 9–11Google Scholar
  12. 12.
    Triger DR, Kurtz JB, MacCallum FO, Wright R (1972) Raised antibody titers to measles and rubella viruses in chronic active hepatitis. Lancet i: 665–667Google Scholar
  13. 13.
    Siegle D, Hirshman SZ (1977) Hepatic dysfunction in acute measles infection of adults. Arch Int Med 137: 1178–1179Google Scholar
  14. 14.
    Vajro P, Hadchouel P, Hadchouel M, Bernard O, Alagille D (1990) Incidence of cirrhosis in children with chronic hepatitis. J Pediatr 117: 392–396Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. Kawashima
    • 1
  • T. Mori
    • 2
  • K. Takekuma
    • 1
  • A. Hoshika
    • 1
  • M. Hata
    • 3
  • T. Nakayama
    • 2
  1. 1.Department of PediatricsTokyo Medical CollegeTokyoJapan
  2. 2.Virology Department, Research DivisionThe Kitasato InstituteTokyoJapan
  3. 3.Department of PediatricsTeikyo UniversityTokyoJapan

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