Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Stimulation of growth of human malignant lymphoma and lymphoid leukemia cells in vitro

Summary

We have analyzed cultures of malignant lymphoma cells and cells from patients with acute lymphoid leukemia in methylcellulose for their ability to from colonies. Clonogenic growth was examined in the presence or absence of fetal calf serum (FCS), platelet-poor plasma (PPP), medium conditioned by phytohemagglutininstimulated leukocytes (PHA-LCM), or irradiated allogeneic bone marrow stroma cells. Cells from 25 lymphoma patients — 17 with non-Hodgkin's lymphoma (NHL), eight with Hodgkin's disease (HD) — and from 19 patients with acute lymphocytic leukemia (ALL) were investigated. We show that colony growth can be obtained in a minority of cases (in 3 NHL, 5 HD, and 2 ALL) and that the use of FCS and allogeneic irradiated stroma cells may be required for optimal colony formation.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Bross KJ, Pangalis GA, Staatz CG (1978) Demonstration of cell surface antigens and their antibodies by the peroxidase-antiperoxidase method. Transplantation 25: 331–334

  2. 2.

    Bross KJ, Schmidt GM, Blume KG, Spruce WE, Farbstein MJ (1979) Confirmation of bone marrow engraftment by demonstration of blood group antigens on red blood cell ghosts. Transplantation 28: 257–259

  3. 3.

    Cicco NA, Oster W, Mertelsmann R, Herrmann F (1992) Growth of malignant B-type lymphoma is stimulated by IL-7. Int J Canc (in press)

  4. 4.

    Coulombel L, Eaves AC, Eaves CJ (1983) Enzymatic treatment of long-term human marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer. Blood 62: 291–297

  5. 5.

    Durie BGM, Young LA, Salmon SE (1983) Human myeloma in vitro colony growth: interrelationship between drug sensitivity, cell kinetics, and patient survival duration. Blood 61: 929–934

  6. 6.

    Epstein AL, Kaplan HS (1979) Feeder layer and nutritional requirements for the establishment and coloning of human malignant lymphoma cell lines. Cancer Res 39: 1748–1759

  7. 7.

    Gartner S, Kaplan HS (1980) Long-term culture of human bone marrow cells. Proc Natl Acad Sci USA 77: 4756–4759

  8. 8.

    Gruss HJ, Brach MA, Drexler HG, Bross KH, Herrmann F (1992) IL-9 is expressed by primary and cultured Hodgkin and Sternberg-Reed cells. Cancer Res (in press)

  9. 9.

    Hamburger AW (1987) The human tumor clonogenic assay as a model system in cell biology. Int J Cell Cloning 5: 89–107

  10. 10.

    Hamburger A, Salmon SE (1977) Primary bioassay of human myeloma stem cells. J Clin Invest 60: 846–854

  11. 11.

    Hamburger AW, Salmon SE (1977) Primary bioassay of human tumor stem cells. Science 197: 461–463

  12. 12.

    Hamburger AW, White CP, Tencer K (1982) Effect of enzymatic disaggregation on proliferation of human tumor cells in soft agar. JNCI 68: 945–949

  13. 13.

    Henss H, Fiebig HH, Meinhardt K, Löhr GW (1984) Clonal growth of human tumor xenografts. First experiences in drug testing. J Cancer Res Clin Oncol 108: 233–235

  14. 14.

    Herrmann F, Schmidt RE, Ritz J, Griffin JD (1987) Regulation of hematopoiesis by NK cells: analysis at the clonal level. Blood 69: 246–253

  15. 15.

    Hoff DD von, Clark GM, Stogdill CBJ, Sarosdy MF, O'Brien MT, Casper JT, Mattox DE, Page CP, Cruz AB, Sandbach JF (1983) Prospective clinical trial of a human tumor cloning system. Cancer Res 43: 1926–1931

  16. 16.

    Oster W, Cicco NA, Klein H, Hirano T, Kishimoto T, Lindemann A, Mertelsmann R, Herrmann F (1989) Participation of the cytokines interleukin-6, tumor necrosis factor-alpha, and interleukin-1 beta secreted by acute myelogenous leukemia blasts in autocrine and paracrine leukemia growth control. J Clin Invest 84: 451–457

  17. 17.

    Rosenberg SA, Berard CW, Brown BW Jr (1982) National Cancer Institute-sponsored study of classifications of non-Hodgkin's lymphomas. Summary and description of a working formulation for clinical usage. Cancer 49: 2112–2135

  18. 18.

    Salmon SE, von Hoff DD (1981) In vitro evaluation of anticancer drugs with the human tumor stem cell assay. Semin Oncol 8: 377–385

  19. 19.

    Shoemaker RH, Wolpert-DeFilippes MK, Kern DH, Lieber MM, Makuch RW, Melnick NR, Miller WT, Salmon SE, Simon RM, Venditti JM, von Hoff DD (1985) Application of a human tumor colony-forming assay to new drug screening. Cancer Res 45: 2145–2153

  20. 20.

    Singletary SE, Umbach GE, Spitzer G, Drewinko B, Tomasovic B, Ajani J, Hug V, Blumenschein G (1985) The human tumor stem cell assay revisited. Int J Cell Cloning 3: 116–128

  21. 21.

    Smith SD, Kisker S, Bush L, Trueworthy RC (1984) Utilization of a tumor cloning system to monitor for bone marrow involvement in children with non-Hodgkin's lymphoma. Cancer 53: 1724–1729

  22. 22.

    Solberg LA, Jamal N, Messner HA (1985) Characterization of human megakaryocyte colony formation in human plasma. J Cell Physiol 124: 67–73

  23. 23.

    Strobel E-S, Gay RE, Greenberg PL (1986) Characterization of the in vitro stromal microenvironment of human bone marrow. Int J Cell Cloning 4: 341–356

  24. 24.

    Strobel E-S, Strobel HG, Bross KJ, Winterhalter B, Fiebig H-H, Schildge JU, Löhr GW (1989) Effects of human bone marrow stroma on the growth of human tumor cells. Cancer Res 49: 1001–1007

  25. 25.

    Tweeddale ME, Lim B, Jamal N, Robinson J, Zalcberg J, Lockwood G, Minden MD, Messner HA (1987) The presence of clonogenic cells in high-grade malignant lymphoma: a prognostic factor. Blood 69: 1307–1314

  26. 26.

    Uyttenhove C, Simpson R, van Smide J (1988) Functional and structural characterization of p40, a mouse glycoprotein with T-cell growth factor activity. Proc Natl Acad Sci USA 85: 8934–8940

Download references

Author information

Correspondence to E. -S. Strobel.

Additional information

This work was supported by theDeutsche Forschungsgemeinschaft (Str 250/2-2).

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Strobel, E.-., Bross, K.J., Mertelsmann, R. et al. Stimulation of growth of human malignant lymphoma and lymphoid leukemia cells in vitro. Ann Hematol 64, 66–71 (1992). https://doi.org/10.1007/BF01715347

Download citation

Key words

  • Human lymphoma clonogenic assay
  • Human bone marrow stroma