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Die Wirkung von Tryptophan-N-Lost auf DNS- und Histonproteingehalt isolierter Tumorzellkerne

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Zusammenfassung

Mit einer einzigen i.v.-Dosis von Tryptophan Mustard, einem neuen N-Lost-Präparat, wurde bei Ratten mit Walker 256 Carcinomsarkom regelmäßig eine Carcinostasis erzeugt. Mikroskopisch zeigte das behandelte Tumorgewebe eine Häufung von großkernigen Riesenzellen und eine Verminderung der Mitosen. Cytophotometrische Messungen an isolierten Feulgen gefärbten Zellkernen vor, 2 und 5Tage nach der Behandlung zeigten eine Verdoppelung des DNS-Gehaltes pro Zellkern bzw. der Ploidiestufen, die bereits am 2. Tag vorhanden war. Mit Fastgreen bei pH 8,1 gefärbte Zellkerne zeigten ebenfalls Verdoppelungen des Histongehaltes nach der Behandlung. Diese Befunde zeigen, daß das alkylierende Mittel in erster Linie die Mitose blockiert, und daß DNS- und Proteinsynthese des Zellkerns nicht primär gehemmt werden.

Summary

Effects of Tryptophan Mustard in vivo on DNA- and Histon-Content of Isolated Tumorcell Nuclei.

A regular carcinostasis was produced in rats bearing the Walker 256 carcinosarcoma by a single i.v. injection of tryptophan mustard. Microscopic examination of the treated tumor revealed an abundance of giant cells with big nuclei and fewer mitotic figures in comparison with the untreated tumor. Cytophotometric measurements of isolated Feulgen stained nuclei before, 2 and 5 days after treatment demonstrated an increased amount of DNA per nucleus with a doubling of the original stem line ploidie. This effect was already complete by day 2 after chemotherapy. A similar behaviour of the histonprotein content per nucleus could be seen after staining with Fastgreen at pH 8.1. These findings seem to underline the specific inhibiting effect of the alkylating agent upon cell division whereas synthesis of nucleic acids and protein is not primarily affected.

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Lampert, F., Sandritter, W. Die Wirkung von Tryptophan-N-Lost auf DNS- und Histonproteingehalt isolierter Tumorzellkerne. Klin Wochenschr 44, 895–898 (1966). https://doi.org/10.1007/BF01711967

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