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25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid

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Summary

Samples of CSF and plasma were obtained simultaneously from 46 adult patients who had no endocrine disorders and were undergoing routine diagnostic lumbar puncture because of suspected or proved prolapse of a disc. Concentrations of 25-OHD, 24,25(OH)2D and 1,25(OH)2D were measured. The samples were purified by column chromatography and fractionated by HPLC. In the appropriate fractions the vitamin D metabolites were measured by PBA, and cytoreceptor assay. The results were as follows (median, range in brackets): 25-OHD in CSF 8.3 ng/ml (2.0–24.8), in plasma 14.5 ng/ml (7.0–36.0). 24,25(OH)2D in CSF 1.8 ng/ml (0.3–4.6) and 2.5 ng/ml (0.4–4.7) in plasma. 1.25(OH)2 D in CSF 25.0 pg/ml (2.2–39.0) and 31.0 pg/ml (10.1–55.0) in plasma. The correlations between plasma and CSF concentrations were as follows: 25-OHDr=0.479 (P<0.001); 24,25(OH)2Dr=0.815 (P<0.001) and for 1.25(OH)2Dr=0.497 (P<0.001).

Our findings showed vitamin D metabolites to be present in human CSF.

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Abbreviations

Ca:

Calcium

CSF:

Cerebrospinal fluid

Vitamin D3 :

Cholecalciferol

CPM:

Counts per min

24, 25 (OH)2D:

24, 25-dihydroxyvitamin D3

1,25(OH)2D:

1,25-dihydroxyvitamin D3

Vitamin D2 :

Ergocalciferol

HPLC:

High-pressure liquid chromatography

25OHD:

25-hydroxyvitamin D3

PTH:

Parathyroid hormone

PBA:

Protein binding assay

RIA:

Radioimmunoassay

D-CaBP:

Vitamin D dependent calcium-binding protein

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Balabanova, S., Richter, H.-., Antoniadis, G. et al. 25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid. Klin Wochenschr 62, 1086–1090 (1984). https://doi.org/10.1007/BF01711378

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Key words

  • Vitamin D metabolites
  • Cerebrospinal fluid