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25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid

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Samples of CSF and plasma were obtained simultaneously from 46 adult patients who had no endocrine disorders and were undergoing routine diagnostic lumbar puncture because of suspected or proved prolapse of a disc. Concentrations of 25-OHD, 24,25(OH)2D and 1,25(OH)2D were measured. The samples were purified by column chromatography and fractionated by HPLC. In the appropriate fractions the vitamin D metabolites were measured by PBA, and cytoreceptor assay. The results were as follows (median, range in brackets): 25-OHD in CSF 8.3 ng/ml (2.0–24.8), in plasma 14.5 ng/ml (7.0–36.0). 24,25(OH)2D in CSF 1.8 ng/ml (0.3–4.6) and 2.5 ng/ml (0.4–4.7) in plasma. 1.25(OH)2 D in CSF 25.0 pg/ml (2.2–39.0) and 31.0 pg/ml (10.1–55.0) in plasma. The correlations between plasma and CSF concentrations were as follows: 25-OHDr=0.479 (P<0.001); 24,25(OH)2Dr=0.815 (P<0.001) and for 1.25(OH)2Dr=0.497 (P<0.001).

Our findings showed vitamin D metabolites to be present in human CSF.

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Cerebrospinal fluid

Vitamin D3 :



Counts per min

24, 25 (OH)2D:

24, 25-dihydroxyvitamin D3


1,25-dihydroxyvitamin D3

Vitamin D2 :



High-pressure liquid chromatography


25-hydroxyvitamin D3


Parathyroid hormone


Protein binding assay




Vitamin D dependent calcium-binding protein


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Balabanova, S., Richter, H.-., Antoniadis, G. et al. 25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid. Klin Wochenschr 62, 1086–1090 (1984).

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Key words

  • Vitamin D metabolites
  • Cerebrospinal fluid