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Infection

, Volume 21, Issue 2, pp 127–130 | Cite as

Thein vitro inactivation of thirteen β-lactam antibiotics by other mechanisms than adsorption to faecal substance

  • H. de Vries-Hospers
  • G. Jansen
  • R. Tonk
  • D. Oenema
  • D. van der Waaij
Originalia

Summary

We have investigated the antibiotic inactivating capacity of intestinal contentsin vitro in faeces. In the presently reported study the influence of β-lactamase catalyzed hydrolysis on the antimicrobial activity of 13 commonly used β-lactam antibiotics was investigated, while the influence of non-specific adsorption of antibiotics to faecal compounds was also taken into account. The following antibiotics were tested: benzylpenicillin, amoxicillin, amoxicillin/clavulanate, cloxacillin, piperacillin, temocillin, cefuroxime, cefamandole, cephradine, cefotaxime, ceftazidime, aztreonam and imipenem. Faecal samples were obtained from 30 healthy volunteers. Six different concentrations of each antibiotic were added to 1 g of faeces. After 24 h of incubation at 37°C the remaining amount of active antibiotic was determined by means of a “growth inhibition assay”. The contribution to the test results of non-specific adsorption to macromolecules was calculated by means of a model and the inactivation data were subsequently corrected. The amount of antibiotic non-specifically bound to faecal macromolecules varied from 0% to 80% of the amount of antibiotic initially added to the faeces. A considerable difference was found in the degree of inactivation of several antibiotics. However, in contrast to earlier investigations, the results of this study show that in a normal population the influence of β-lactamase catalyzed hydrolysis on the activity of β-lactam antibiotics is apparently very small when compared to the influence of non-specific adsorption of β-lactam antibiotics to faecal compounds.

Keywords

Ceftazidime Imipenem Cefuroxime Piperacillin Aztreonam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Inaktivierung von 13 β-Laktamantibiotika durch Mechanismen, die nicht auf Adsorption durch Bestandteile der Faeces zurückzuführen sind

Zusammenfassung

Die Fähigkeit des Darminhaltes, Antibiotika zu inaktivieren, wurdein vitro untersucht. In der vorliegenden Studie wurde neben der unspezifischen Adsorption von Antibiotika an Stuhlbestandteile vor allem der Einfluß der β-Laktamase-vermittelten Hydrolyse auf die anti-mikrobielle Aktivität von 13 gebräuchlichen β-Laktamantibiotika untersucht (Benzylpenicillin, Amoxicillin, Amoxicillin/Clavulansäure, Cloxacillin, Piperacillin, Temocillin, Cefuroxim, Cefamandol, Cephradin, Cefotaxim, Ceftazidim, Aztreonam und Imipenem). Die Stuhlproben wurden von 30 gesunden Probanden gewonnen. Jedes Antibiotikum wurde in sechs verschiedenen Konzentrationen jeweils zu 1 g Faeces gegeben. Nach 24 h Inkubationszeit bei 37°C wurde mittels „Wachstumshemmtest“ die Restaktivität des Antibiotikums bestimmt. Die Ergebnisse wurden mit dem Faktor der unspezifischen Adsorption an Makromoleküle (Berechnung nach entsprechendem Modell) korrigiert. Von der ursprünglich zugegebenen Antibiotikamenge wurden 0 bis 80% unspezifisch an Makromoleküle der Faeces gebunden. Zwischen dem Inaktivierungsgrad der Antibiotika bestanden beträchtliche Unterschiede. Im Vergleich zur unspezifischen Adsorption an Stuhlbestandteile erwies sich in dieser Studie — im Gegensatz zu früheren Untersuchungen — der Einfluß der Hydrolyse durch β-Laktamasen auf die Antibiotikaaktivität bei gesunden Personen als sehr gering.

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Copyright information

© MMV Medizin Verlag GmbH München 1993

Authors and Affiliations

  • H. de Vries-Hospers
    • 1
  • G. Jansen
    • 1
  • R. Tonk
    • 1
  • D. Oenema
    • 1
  • D. van der Waaij
    • 1
  1. 1.Laboratory of Medical Microbiology, Oostersingel 59, Entrance 21University Hospital GroningenGroningenThe Netherlands

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