Intensive Care Medicine

, Volume 17, Issue 1, pp 30–35 | Cite as

Quantitative blood cultures for diagnosis and management of catheter-related sepsis in pediatric hematology and oncology patients

  • M. C. Douard
  • G. Arlet
  • G. Leverger
  • R. Paulien
  • C. Waintrop
  • E. Clementi
  • B. Eurin
  • G. Schaison


Paired quantitative blood cultures collected simultaneously via catheter and peripheral vein in Isolator 1.5 ml tubes, were performed in 50 febrile hematology children. Samples were taken to diagnose catheter-related sepsis (CRS) without catheter removal and to monitor the therapeutic efficiency of antimicrobials administered through the infected device by infusion and/or by the antibiotic lock technique (ALT). In 7 children (14%) the colony counts from catheter blood samples were 30-fold higher than the colony counts from peripheral samples, suggesting CRS; in 7 other patients (14%), identical colony counts in both samples suggested sepsis was not catheter-related. One patient (2%) had septicemia caused byE. coli found in the urinary tract; only the peripheral blood cultures were positive. In 6 patients (12%), the Isolator system was not effective for diagnosing bacteremia or CRS; in 29 patients (58%) the febrile episode was not microbiologically documented. All episodes of CRS were cured whatever the treatment was: infusion or ALT.

Key words

Quantitative blood cultures Catheter-related sepsis Antibiotic lock technique 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • M. C. Douard
    • 1
  • G. Arlet
    • 2
  • G. Leverger
    • 3
  • R. Paulien
    • 1
  • C. Waintrop
    • 1
  • E. Clementi
    • 1
  • B. Eurin
    • 1
  • G. Schaison
    • 3
  1. 1.Departement d'AnesthesiologieHôpital Saint-LouisParisFrance
  2. 2.Laboratoire de Microbiologie et de VirologieHôpital Saint-LouisParisFrance
  3. 3.Unité d'Hématologie pédiatriqueHôpital Saint-LouisParisFrance

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