Lack of correlation between vacuolating cytotoxin activity,cagA gene inHelicobacter pylori, and peptic ulcer disease in children
- 30 Downloads
Abstract
To determine the prevalence of thecagA gene and vacuolating cytotoxin inHelicobacter pylori isolates obtained from children and to characterize the relationship betweencagA, cytotoxin production, and ulcerogenesis, pediatricHelicobacter pylori isolates were tested forcagA by the polymerase chain reaction and for vacuolating cytotoxin by a cell culture assay.Helicobacter pylori isolates were obtained from 33 children referred for upper gastrointestinal endoscopy. Twenty-six of these isolates were tested forcagA by the polymerase chain reaction; all 26 (100%) were positive. Of the 26 children from whom these isolates were obtained, 26 (100%) had chronic gastritis and 12 (46%) had duodenal ulcers. Nine (30%) of 30 isolates tested showed expression of vacuolating cytotoxin, only three of which came from patients with duodenal ulceration (odds ratio 0.81, 95% confidence interval 0.1–5.3). Of the 23cagA-positive isolates tested for cytotoxin, only nine (39%) were positive. There was no association between vacuolating cytotoxin and clinical symptoms, nor was cytotoxicity associated with ulcerogenesis. In summary, the findings suggest thatcagA is not a marker of duodenal ulceration or of vacuolating cytotoxin production in children referred for endoscopy.
Keywords
Polymerase Chain Reaction Cell Culture Internal Medicine Clinical Symptom Peptic UlcerPreview
Unable to display preview. Download preview PDF.
References
- 1.Blaser MJ:Helicobacter pylori phenotypes associated with peptic ulceration. Scandinavian Journal of Gastroenterology (1994) 29, Supplement 205:1–5Google Scholar
- 2.Weel JFL, van der Hulst RWM, Gerritis Y, Roorda P, Feller M, Dankert J, Tytgat GNJ, van der Ende A: The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, andHelicobacter pylori — related diseases. Journal of Infectious Diseases (1996) 173:1171–1175Google Scholar
- 3.Figura N, Guglielmetti P, Rossolini A, Barberi A, Cusi G, Musmanno RA, Russi M, Quaranta S: Cytotoxin production byCampylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. Journal of Clinical Microbiology (1989) 27:225–226Google Scholar
- 4.Tee W, Lambert JR, Dwyer B: Cytotoxin production byHelicobacter pylori from patients with upper gastrointestinal tract disease. Journal of Clinical Microbiology (1995) 33:1203–1205Google Scholar
- 5.Garner JA, Cover T: Analysis of genetic diversity in cytotoxin-producing and non-cytotoxin producingHelicobacter pylori strains. Journal of Infectious Diseases (1995) 172:290–293Google Scholar
- 6.Peura DA: The report of the Digestive Health Initiative International Update Conference onHelicobacter pylori. Gastroenterology (1997) 113, Supplement 6:4–8Google Scholar
- 7.Husson MO, Gottrand F, Vachee A, Dhaenens L, Martin de la Salle E, Turck D, Houcke M, Leclerc H: Importance in diagnosis of gastritis of detection by PCR of thecagA gene inHelicobacter pylori strains isolated from children. Journal of Clinical Microbiology (1995) 33:3300–3303Google Scholar
- 8.Gzyl A, Berg DE, Dzierzanowska D: Epidemiology of cagA/ vacA genes inHelicobacter pylori isolated from children and adults in Poland. Journal of Physiology and Pharmacology (1997) 48:333–343Google Scholar
- 9.Moore DD: Preparation of genomic DNA from bacteria. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds): Short protocols of molecular biology. New York (1992) pp. 210–212Google Scholar
- 10.Xiang Z, Censini S, Bayeli PF, Telford JL, Figura N, Rappuoli R, Covacci A: Analysis of expression ofcagA andvacA virulence factors in 43 strains ofHelicobacter pylori reveals that clinical isolates can be divided into two major types and thatcagA is not necessary for expression of the vacuolating cytotoxin. Infection and Immunity (1995) 63:94–98Google Scholar
- 11.Leunk RD, Johnson PT, David BC, Kraft WG, Morgan DR: Cytotoxic activity in broth culture filtrates ofCampylobacter pylori. Journal of Medical Microbiology (1988) 26:93–99Google Scholar
- 12.Pan ZJ, van der Hulst RWM, Feller M, Xiao S, Tytgat GNJ, Dankert J, van der Ende A: Equally high prevalences of infection with cagA-positiveHelicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis-associated dyspepsia. Journal of Clinical Microbiology (1997) 35:1344–1347Google Scholar
- 13.Shimoyama T, Fukuda S, Tanaka M, Mikami T, Saito Y, Munakata A: High prevalence of thecagA -positiveHelicobacter pylori strains in Japanese asymptomatic patients and gastric cancer patients. Scandinavian Journal of Gastroenterology (1997) 32:465–468Google Scholar