The influence of various sera and proteins on the uptake of a superparamagnetic colloid (magnetic starch microspheres (MSM); particle size, 200 nm; crystal size, 10 nm) by the isolated and perfused rat liver has been studied. It is demonstrated that the capture of MSM is slightly reduced by the addition of rat blood to the protein-free perfusion medium but highly reduced by newborn calf serum (NCS). The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis of proteins adsorbed on the nanoparticles incubated in NCS reveals major coating by albumin and IgG. The addition of bovine IgG to the perfusion fluid reduces the rate of MSM uptake in the same extent that NCS, whereas fetal calf serum that contains only traces of IgG weakly alters the MSM clearance. Finally, complemented and decomplemented NCS exhibit the same influence on the MSM hepatic extraction. It is concluded that although lectins are largely involved in the uptake of MSM administered in the absence of proteins, opsonins receptors are implicated when the perfusion medium contains relevant blood components.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Weissleder R, Papisov M (1992) Pharmaceutical Iron Oxides for MR Imaging.Reviews of Magnetic Resonance in Medicine 4 1.
Roch A, Bach-Gansmo T, Muller RN (1993) In vitro relaxometric characterization of superparamagnetic contrast agents.MAGMA 1 83.
Fahlvik AK, Holtz E, Leander P, Schroder U, Klaveness J (1990) Magnetic starch microspheres, efficacy and elimination. A new organ-specific contrast agent for magnetic resonance imaging.Invest Radiol 25 113.
Colet JM, Van Haverbeke Y, Muller RN (1994) Evidences for attachment of magnetic starch microspheres to Kupffer cells receptors in excised and perfused rat liver.Invest Radiol.29: S223.
Harashima H, Ohnishi Y, Kiwada H (1992) In vivo evaluation of the effect of the size and the opsonization on the hepatic extraction of liposomes in rats: an application of Oldendorf method.Biopharm Drug Disp 13 549.
Harashima H, Sakata K, Kiwada H (1993) Distinction between the depletion of opsonins and the saturation of uptake in the dose-dependent hepatic uptake of liposomes.Pharma Res 10 606.
Artursson P, Sjöholm I (1986) Effect of opsonins on the macrophage uptake of polyacrylstarch microparticles.Int JPharm 32 165.
Artursson P, Johansson D, Sjöholm I (1988) Receptor-mediated uptake of starch and mannan microparticles by macrophages: Relative contribution of receptors for complement, immunoglobulins and carbohydrates.Biomaterials 9 241.
About this article
Cite this article
Colet, J., Muller, R.N. Effect of opsonins on the uptake of magnetic starch microspheres by rat Kupffer cells. MAGMA 2, 303–305 (1994). https://doi.org/10.1007/BF01705258
- MRI contrast agents
- superparamagnetic particles
- perfused liver