Advertisement

Virus Genes

, Volume 7, Issue 3, pp 255–264 | Cite as

Comparative sequence analysis of the coat proteins of biologically distinct citrus tristeza closterovirus isolates

  • Hanu PappuEmail author
  • Sita Pappu
  • Chuck Niblett
  • Richard Lee
  • Ed Civerolo
Article

Abstract

The genome of citrus tristeza closterovirus (CTV) consists of a 20 kb single-stranded RNA encapsidated in a 2000 nm long, flexuous particle. Double-stranded (replicative form) RNAs purified from CTV-infected tissue were used to prepare complementary DNA libraries that involved initial first-strand cDNA synthesis followed by selective amplification of the coat protein gene. CTV-specific antisera were used to select clones expressing the coat protein. The coat protein genes of seven Florida and four exotic isolates that differ in their biological properties were cloned and sequenced. The gene is 669 base pairs long and encodes a 223 amino acid protein. There was a greater than 80% homology at both nucleotide and amino acid levels among all the isolates examined. However, comparisons showed that each isolate was found to have several unique amino acid residues. Several blocks of amino acid residues were conserved among all the isolates. A cluster dendrogram showed greater similarities among groups of mild and severe Florida isolates that differed significantly from those of the geographically distinct, exotic CTV isolates.

Key words

citrus tristeza closterovirus amino acid sequence 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bar-Joseph M. and Lee, R.F., Commonwealth Mycological Institute/Association of Applied Biologists: Description of Plant Viruses. No. 353, 1990.Google Scholar
  2. 2.
    Bar-Joseph M., Garnsey S.M., and Gonsalves D., Adv Virus Res25 93–168, 1979.Google Scholar
  3. 3.
    Bar-Joseph M., Marcus R., and Lee R.F., Annu Rev Phytopathol27 292–316, 1989.Google Scholar
  4. 4.
    Dodds J.A., Jordan R.L., Roistacher C.N., and Jarupat T., Intervirology27 177–188, 1987.Google Scholar
  5. 5.
    Valverde R.A., Dodds J.A., and Heick J.A., Phytopathology76 459–465, 1986.Google Scholar
  6. 6.
    Lee R.F., Proc Fla State Hort Soc97 53–56, 1984.Google Scholar
  7. 7.
    Yokomi R.K. and Garnsey S.M., Phytophylactica19 169–172, 1987.Google Scholar
  8. 8.
    Lee R.F., Brlansky R.H., Garnsey S.M., and Yokomi R.K., Phytophylactica19 215–218, 1987.Google Scholar
  9. 9.
    Broadbent P., Bevington K.B., and Coot B.G., Proc. 11 Conf. Intl. Organ. Citrus Virol., Riverside, CA, 1991, pp. 64–70.Google Scholar
  10. 10.
    Beachy R.N., Loesch-Fries S., and Tumer N.E., Annu Rev Phytopathol28 451–474, 1990.Google Scholar
  11. 11.
    Dulieu P. and Bar-Joseph M., J Gen Virol71 443–447, 1990.Google Scholar
  12. 12.
    Niblett C.L., Stark D.M., Lee R.F., and Beachy R.N., Phytopathology81 698, 1991.Google Scholar
  13. 13.
    Sekiya M.E., Lawrence S.D., McCaffery M., and Cline K., J Gen Virol72 1013–1020, 1991.Google Scholar
  14. 14.
    Pappu H.R., Lee R.F., Pappu S.S., Niblett C.L., and Karkashian J., Proc. Australian Plant Pathol. Soc., p. 23, 1991.Google Scholar
  15. 15.
    Rosner A., Lee R.F., and Bar-Joseph M., Phytopathology76 820–824, 1986.Google Scholar
  16. 16.
    Yokomi R.K., Garnsey S.M., Permar T.A., Lee R.F., and Youtsey C.O. in Proc. 11 Conf. Int. Organ. Citrus Virologists, Riverside, CA, pp. 86–92, 1991.Google Scholar
  17. 17.
    Rocha-Pena M.A., Lee R.F., Permar T.A., Yokomi R.K., and Garnsey S.M. in Proc. 11 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 93–102, 1991.Google Scholar
  18. 18.
    Van Vuuren S.P. and Moll J.N., Phytophylactica19 219–221, 1987.Google Scholar
  19. 19.
    Ballester-Olmos J.F., Pina J.A., and Navarro L. in Proc. 10 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 28–32, 1988.Google Scholar
  20. 20.
    Kano T., Garnsey S.M., Koizumi M., and Permar T.A. in Proc. 11 Conf. Intl. Organ. Citrus Virologists, Riverside, CA, pp. 51–55, 1991.Google Scholar
  21. 21.
    Morris T.J. and Dodds J.A., Phytopathology69 854–858, 1979.Google Scholar
  22. 22.
    Sambrook J., Fritsch E.F., and Maniatis T.,Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY, 1989.Google Scholar
  23. 23.
    Vieira J. and Messing J., Meth Enzymol153 3–11, 1989.Google Scholar
  24. 24.
    Still P.E., Hunter T.J., Rocha-Pena M.A., Lee R.F., and Niblett C.L., Phytopathology81 695, 1991.Google Scholar
  25. 25.
    Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res12 387–395, 1984.Google Scholar
  26. 26.
    Rhoads D.D. and Roufa D.S., Mol Cell Biol5 1655–1659, 1985.Google Scholar
  27. 27.
    Garnsey S.M., Gumpf D.J., Roistacher C.N., Civerolo E.L., Lee R.F., Yokomi R.K., and Bar-Joseph M., Phytophylactica19 151–157, 1987.Google Scholar
  28. 28.
    Permar T.A., Garnsey S.M., Gumpf D.J., and Lee R.F., Phytopathology80 224–228, 1990.Google Scholar
  29. 29.
    Garnsey S.M., Kano T., Permar T.A., Cambra M., Koizumi M., and Vela C., Phytopathology79 1174, 1989.Google Scholar
  30. 30.
    Dawson W.O., Virology186 359–367, 1992.Google Scholar
  31. 31.
    Garnsey S.M., Civerolo E.L., Gumpf D.J., Yokomi R.K., and Lee R.F. in Proc. 11th Intl. Org. Citrus Virol., Riverside, CA, 1991, pp. 113–120.Google Scholar
  32. 32.
    Needleman S.B. and Wunsch C.D., J Mol Biol48 443–453, 1970.Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Hanu Pappu
    • 1
    Email author
  • Sita Pappu
    • 1
  • Chuck Niblett
    • 1
  • Richard Lee
    • 2
  • Ed Civerolo
    • 3
  1. 1.Plant Pathology DepartmentUniversity of FloridaGainesvilleUSA
  2. 2.Citrus Research and Education CenterUniversity of FloridaLake Alfred
  3. 3.United States Department of AgricultureAgricultural Research ServiceBeltsville

Personalised recommendations