Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

  • S. Cruchaga D'Harcourt
  • A. Buño Soto
  • V. Cascante Burgos
  • D. Lozano Calero
  • R. Martínez-Zapico
Notes

Abstract

To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.

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References

  1. 1.
    Derrick EH: Q fever, a new fever entity: clinical features, diagnosis and laboratory investigation. Medical Journal of Australia 1937, 2: 281–299.Google Scholar
  2. 2.
    Marrie TJ:Coxiella burnetii (Q fever). In: Mandell GL, Douglas RGJ, Bennett JE (ed): Principles and practice of infectious diseases. Churchill Livingstone, New York, 1995, p. 1727–1735.Google Scholar
  3. 3.
    Raoult D, Marrie T: Q fever. Clinical Infectious Diseases 1995, 20: 489–496.PubMedGoogle Scholar
  4. 4.
    Peacock MG, Philip RN, Williams MS, Faulkner RS: Serological evaluation of Q fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis. Infection and Immunity 1983, 41: 1089–1098.PubMedGoogle Scholar
  5. 5.
    Roges G, Edlinger E: Immunoenzymatic test for Q fever. Diagnostic Microbiology and Infectious Diseases 1986, 4: 125–132.Google Scholar
  6. 6.
    Behymer DE, Ruppanner R, Brooks D, Williams JC, Franti CE: Enzyme immunoassay for surveillance of Q fever. American Journal of Veterinary Research 1985, 46: 2413–2417.PubMedGoogle Scholar
  7. 7.
    Field PR, Hunt JG, Murphy AM: Detection and persistence of specific IgM antibody toCoxiella burnetii by enzyme-linked immunosorbent assay. A comparison with immunofluorescence and complement-fixation tests. Journal of Infectious Diseases 1983, 148: 477–487.PubMedGoogle Scholar
  8. 8.
    Peter O, DuPuis M, Peacock MG, Burgdorfer W: Comparison of enzyme-linked immunosorbent assay and complement fixation and indirect fluorescent-antibody test for detection ofCoxiella burnetii antibody. Journal of Clinical Microbiology 1987, 25: 1063–1067.PubMedGoogle Scholar
  9. 9.
    Cowley R, Fernandez F, Freemantle W, Rutter D: Enzyme immunoassay for Q fever: comparison with complement fixation and immunofluorescence test and dot immunoblotting. Journal of Clinical Microbiology 1992, 30: 2451–2455.PubMedGoogle Scholar
  10. 10.
    Roges G, Edlinger E: Immunoenzymatic test for Q-fever. Diagnostic Microbiology and Infectious Diseases 1986, 4: 125–132.Google Scholar
  11. 11.
    Uhaa IJ, Fishbein DB, Olson JG, Rives CC, Waag DM, Williams JC: Evaluation of specificity of indirect enzymelinked immunosorbent assay for diagnosis of human Q fever. Journal of Clinical Microbiology 1994, 32: 1560–1565.PubMedGoogle Scholar
  12. 12.
    Döller G, Döller PC, Gerth HJ: Early diagnosis of Q fever: detection of immunoglobulin M by radioimmunoassay and enzyme immunoassay. European Journal of Clinical Microbiology 1984, 3: 550–553.PubMedGoogle Scholar
  13. 13.
    Peter O, Dupuis G, Bee D, Luthy R, Nicolet J, Burgdorfer W: Enzyme-linked immunosorbent assay for diagnosis of chronic Q fever. Journal of Clinical Microbiology 1988, 26: 1978–1982.PubMedGoogle Scholar
  14. 14.
    Tellez A, Martin A, Anda P, de la Fuente L, Benitez P, García C, León P: Study ofCoxiella burnetii human and animal seroprevalence in a rural population in Madrid community. European Journal of Epidemiology 1989, 5: 444–446.PubMedGoogle Scholar
  15. 15.
    Ruiz-Beltrán R, Herrero-Herrero JI, Martín-Sánchez AM, Martín-González JA: Prevalence of antibodies toRickettsia conorii, Coxiella burnetii, andRickettsia typhi in Salamanca province (Spain). Serosurvey in the human population. European Journal of Epidemiology 1990, 6: 293–299.PubMedGoogle Scholar

Copyright information

© MMV Medizin Verlag GmbH 1996

Authors and Affiliations

  • S. Cruchaga D'Harcourt
    • 1
  • A. Buño Soto
    • 1
  • V. Cascante Burgos
    • 1
  • D. Lozano Calero
    • 1
  • R. Martínez-Zapico
    • 1
  1. 1.Microbiology DepartmentLa Paz Universitary HospitalMadridSpain

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