Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

  • S. Cruchaga D'Harcourt
  • A. Buño Soto
  • V. Cascante Burgos
  • D. Lozano Calero
  • R. Martínez-Zapico


To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.


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Copyright information

© MMV Medizin Verlag GmbH 1996

Authors and Affiliations

  • S. Cruchaga D'Harcourt
    • 1
  • A. Buño Soto
    • 1
  • V. Cascante Burgos
    • 1
  • D. Lozano Calero
    • 1
  • R. Martínez-Zapico
    • 1
  1. 1.Microbiology DepartmentLa Paz Universitary HospitalMadridSpain

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