Clinical evaluation of a commercial ligase-based gene amplification method for detection ofMycobacterium tuberculosis

  • P. Alonso
  • A. Orduña
  • M. A. Bratos
  • A. San Miguel
  • A. Rodríguez Torres


The purpose of this study was to evaluate the clinical usefulness of a commercial ligase-based gene amplification method (LCxMycobacterium tuberculosis test; Abbott Laboratories, USA) for detection ofMycobacterium tuberculosis. The tuberculosis infection rate among clinical samples was 10.6%. The sensitivity, specificity, and positive and negative predictive values were 23.5%, 100%, 100%, and 91.7%, respectively, with the fluorochrome auramine stain; 32.4%, 100%, 100%, and 92.6%, respectively, with culture; and 76.5%, 95.8%, 68.4% and 97.2%, respectively, with the gene amplification method. When only samples from patients without current or previous treatment were studied, the sensitivity was 36.4% with the auramine stain, 63.6% with culture, and 100% with the gene amplification assay. The mean treatment time for culture-negative and assay-negative samples was greater than that of culture-negative and assay-positive samples. The LCxMycobacterium tuberculosis test is a sensitive method for detection and identification ofMycobacterium tuberculosis. It produces few false-positive results. However, as it can remain positive after the culture becomes negative, it is not recommended for evaluation of treatment efficiency.


Internal Medicine Tuberculosis Clinical Evaluation Infection Rate Treatment Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • P. Alonso
    • 1
  • A. Orduña
    • 2
  • M. A. Bratos
    • 2
  • A. San Miguel
    • 1
  • A. Rodríguez Torres
    • 2
  1. 1.Central LaboratoryRegional HospitalLugoSpain
  2. 2.Microbiology ServiceUniversity Teaching Hospital, Faculty of MedicineValladolidSpain

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